TY - JOUR AB - Coronary atherosclerotic heart disease poses a significant threat to human health. The pathological basis is atherosclerosis and foam‑cell formation is the key factor in the initiation of atherosclerosis. In the present study, foam cell models were established using 50 ng/ml oxidized low‑density lipoprotein to stimulate in vitro cultures of THP‑1 cells for 72 h. The expression of zinc finger protein 580 (ZNF580), a Cys2‑His2 zinc finger protein containing 172 amino acids that was originally cloned by screening a human aortic cDNA library, was measured in foam cells and its interaction with various regulatory factors during foam‑cell formation was investigated. Oil red O staining was used to observe cell morphology and intracellular lipid levels. Lentivirus transfection was employed to either overexpress or silence ZNF580 in THP‑1 cells, and an inverted fluorescence microscope was used to observe the distribution of ZNF580 immunofluorescence to determine the transfection rate. RNA and total protein were extracted and the expression levels of ZNF580, CD36, peroxisome proliferator‑activated receptor‑γ (PPAR‑γ), ATP‑binding cassette transporter A1 (ABCA1) and apolipoprotein E (ApoE) were measured by reverse transcription‑quantitative PCR. The protein levels were examined by western blot analysis to evaluate the interaction between ZNF580 and associated regulatory factors. ZNF580 was able to significantly increase the expression levels of ApoE and ABCA1 and significantly decrease the expression levels of CD36 and PPAR‑γ, suggesting that ZNF580‑mediated inhibition of foam‑cell formation is associated with the PPAR‑γ‑CD36 signalling pathway. Based on these findings, ZNF580 may be a potential therapeutic candidate for the treatment of coronary atherosclerotic heart disease. AD - The Fourth Detachment, China Coast Guard, Wenchang, Hainan 571300, P.R. China Department of Nephrology with Integrated Traditional Chinese and Western Medicine, No. 2 People's Hospital of The Three Gorges University, Yichang, Hubei 443000, P.R. China Department of Dermatology, No. 923 Hospital of Joint Logistics Support Force, PLA, Nanning, Guangxi 530021, P.R. China Department of Pharmacy, Heilongjiang Municipal Corps Hospital of Chinese People's Armed Police Force, Harbin, Heilongjiang 150076, P.R. China Department of Anesthesia, Hainan Hospital of PLA General Hospital, Sanya, Hainan 572013, P.R. China Department of Health Service, Logistics University of People's Armed Police Force, Tianjin 300309, P.R. China Faculty of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, SAR 214000, P.R. China Department of Cardiac Thoracic Surgery, Characteristic Medical Center of People's Armed Police Force, Tianjin 300309, P.R. China Department of Intensive Care Unit, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China Department of Psychology, Heilongjiang Municipal Corps Hospital of Chinese People's Armed Police Force, Harbin, Heilongjiang 150076, P.R. China AU - Zhang,Zhongbai AU - Qin,Xueting AU - Liu,Jiyuan AU - Li,Yanchun AU - Chen,Huaxin AU - Xie,Hongwei AU - Chen,Jingxun AU - Li,Chuang AU - Tong,Yang AU - Yang,Min AU - Zhang,Mei DA - 2022/09/01 DO - 10.3892/etm.2022.11516 IS - 3 JO - Exp Ther Med KW - ZNF580 cardiovascular disease atherosclerosis foam cells PY - 2022 SN - 1792-0981 1792-1015 SP - 579 ST - Role and mechanism of the zinc finger protein ZNF580 in foam‑cell formation T2 - Experimental and Therapeutic Medicine TI - Role and mechanism of the zinc finger protein ZNF580 in foam‑cell formation UR - https://doi.org/10.3892/etm.2022.11516 VL - 24 ER -