TY - JOUR AB - Gastric cancer (GC) is one of the commonest malignant tumors of the digestive system, characterized by high morbidity and mortality rates. It has been reported that NOD like receptor (NLR) family, CARD domain containing 5 (NLRC5) serves an important role in the occurrence and development of GC. Therefore, the current study aimed to investigate the role of NLRC5 in GC. The mRNA and protein expression levels of NLRC5 in GC cell lines were determined by reverse transcription‑quantitative PCR and western blot analysis, respectively. Additionally, following NLRC5 knockdown, cell proliferation, invasion and migration were evaluated using Cell Counting Kit 8, colony formation, wound healing and Transwell assays, and western blot analysis. The NLRC and Yin Yang 1 (YY1) expression in the AGS cells with 5‑FU resistance were detected by western blotting. The sensitivity of GC cells to 5‑fluorouracil (5‑FU) was detected by flow cytometry and western blot analysis. Additionally, the binding capacity of YY1 on NLRC5 promoter was predicted using JASPAR database and it was further verified by chromatin immunoprecipitation and luciferase reporter assays. Finally, to elucidate the mechanism underlying the effect of NLRC5 on GC, YY1 was overexpressed and NLRC5 was silenced in GC cell lines. The results showed that NLRC5 was abnormally upregulated in GC cells. In addition, NLRC5 knockdown significantly attenuated the proliferation, invasion and migration abilities of GC cells, while it enhanced the sensitivity of GC cells to 5‑FU. The above effects were regulated by the YY1 transcription factor. Overall, the results of the present study indicated that NLRC5 silencing could reduce the malignant growth and enhance the sensitivity of GC cells to 5‑FU chemotherapy via inhibiting the carcinogenic effect of YY1. AD - College of Modern Agriculture and Bioengineering, Yangtze Normal University, Chongqing 408000, P.R. China AU - Liang,Shan AU - Xiang,Tingting AU - Liu,Shiyu AU - Xiang,Wei DA - 2022/09/01 DO - 10.3892/etm.2022.11538 IS - 3 JO - Exp Ther Med KW - NOD like receptor family CARD domain containing 5 Yin Yang 1 gastric cancer malignant growth 5‑fluorouracil PY - 2022 SN - 1792-0981 1792-1015 SP - 601 ST - Inhibition of NLRC5 attenuates the malignant growth and enhances the sensitivity of gastric cancer cells to 5‑FU chemotherapy by blocking the carcinogenic effect of YY1 T2 - Experimental and Therapeutic Medicine TI - Inhibition of NLRC5 attenuates the malignant growth and enhances the sensitivity of gastric cancer cells to 5‑FU chemotherapy by blocking the carcinogenic effect of YY1 UR - https://doi.org/10.3892/etm.2022.11538 VL - 24 ER -