TY - JOUR
AB - Epidemiological studies have suggested an association between obesity and periodontal disease. Brown adipose tissue (BAT) has an anti‑obesity effect. However, the effects of periodontitis on obesity and BAT remain unclear. Therefore, the present study aimed to determine the effects of lipopolysaccharide derived from Porphyromonas gingivalis (P. gingivalis LPS) on brown adipocytes. For this purpose, the present study examined the effects of the intravenous administration of Porphyromonas gingivalis (P. gingivalis) in mice, the treatment of brown adipocytes with P. gingivalis LPS during differentiation, and the administration of small interfering RNA targeting interferon on brown preadipocytes by assessing the expression of genes involved in differentiation, using a long non‑coding (lnc)RNA, and pro‑inflammatory factors using reverse transcription‑quantitative PCR. In addition, the accumulation of lipid droplets was examined using Oil Red O staining. P. gingivalis LPS reduced the expression of uncoupling protein 1 (UCP1) and lncRNA‑BATE10 in brown adipocytes during differentiation. Consistent with this finding, P. gingivalis reduced UCP1 and lncRNA‑BATE10 expression in the BAT of mice. lncRNA‑BATE10 may thus be involved in the regulation of UCP1 expression that occurs during the differentiation of brown adipocytes treated with P. gingivalis LPS. Thus, P. gingivalis LPS may inhibit BAT differentiation by reducing lncRNA‑BATE10 expression.
AD - Department of Stomatology, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, Zhejiang 311202, P.R. China
Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, Zhejiang 310000, P.R. China
Department of Stomatology, Emergency General Hospital, Beijing 100028, P.R. China
AU - Zheng,Faxiang
AU - Su,Lingkai
AU - Zhang,Ning
AU - Liu,Longjv
AU - Gu,Jingyi
AU - Du,Wenhua
DA - 2022/12/01
DO - 10.3892/etm.2022.11654
IS - 6
JO - Exp Ther Med
KW - Porphyromonas gingivalis LPS
brown adipose tissue
lncRNA
inflammation
PY - 2022
SN - 1792-0981
1792-1015
SP - 718
ST - Porphyromonas gingivalis‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10
T2 - Experimental and Therapeutic Medicine
TI - Porphyromonas gingivalis‑derived lipopolysaccharide inhibits brown adipocyte differentiation via lncRNA‑BATE10
UR - https://doi.org/10.3892/etm.2022.11654
VL - 24
ER -