TY - JOUR AB - The current study aimed to determine the expression of carnitine palmitoyltransferase 1A (Cpt1a) in the lung tissue of chronic obstructive pulmonary disease (COPD) patients and its correlation with lung function. An increase in Cpt1a expression improved lung function in patients with COPD by inhibiting apoptosis and the inflammatory response of lung endothelial cells. Lung tissues of 20 patients with COPD and 10 control patients were collected, their Cpt1a expression was determined by western blotting and apoptosis and inflammation were assessed by haematoxylin‑eosin staining, TUNEL assay and ELISA. Mice with knockout or overexpression of Cpt1a were constructed by lentivirus in vivo. A COPD model was induced by cigarette smoke and the role of Cpt1a in COPD was determined in vivo and in vitro. Cpt1a expression was positively correlated with lung function and negatively correlated with apoptosis and inflammation. Patients with COPD with higher expression of Cpt1a in lung tissues had improved lung function indices and lung tissue morphology with less apoptosis and decreased inflammatory response. Compared with the control group, COPD mice with Cpt1a knockdown had aggravated lung dysfunction and increased lung inflammation and apoptosis. Overexpression of Cpt1a alleviated lung dysfunction and reduced inflammatory response and apoptosis of lung tissues in COPD mice. Pulmonary microvascular endothelial cells of mice were isolated in vitro and the results were consistent with the findings obtained in vivo. In conclusion, the clinical, in vivo and in vitro data confirmed for the first time that Cpt1a alleviated lung dysfunction of patients with COPD by inhibiting apoptosis of endothelial cells and inflammatory responses. AD - Department of Respiratory and Critical Care Medicine, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China School of Basic Medical Sciences, Department of Pharmacology, Shanxi Medical University, Taiyuan 030000, P.R. China Department of R&D, USBAY Biotechnology Co., Ltd, Beijing 102006, P.R. China AU - Li,Lifang AU - Zhang,Yaqian AU - Gong,Jiannan AU - Yang,Guang AU - Zhi,Shuyin AU - Ren,Dongping AU - Zhao,Hui AU - Li,Lifang AU - Zhang,Yaqian AU - Gong,Jiannan AU - Yang,Guang AU - Zhi,Shuyin AU - Ren,Dongping AU - Zhao,Hui DA - 2023/01/01 DO - 10.3892/etm.2022.11753 IS - 1 JO - Exp Ther Med KW - chronic obstructive pulmonary disease carnitine palmitoyltransferase 1A apoptosis inflammation PY - 2023 SN - 1792-0981 1792-1015 SP - 54 ST - Cpt1a alleviates cigarette smoke‑induced chronic obstructive pulmonary disease T2 - Experimental and Therapeutic Medicine TI - Cpt1a alleviates cigarette smoke‑induced chronic obstructive pulmonary disease UR - https://doi.org/10.3892/etm.2022.11753 VL - 25 ER -