TY - JOUR AB - Colorectal cancer (CRC) is one of the most common cancers worldwide and the consumption of a high‑calorie diet is one of its risk factors. Calorie restriction (CR) slows tumor growth in a variety of cancers, including colorectal cancer; however, the mechanism behind this remains unknown. In the present study, CR effectively reduced the tumor volume and weight in a xenograft BALB/c male nude mouse model. In addition, tumor immunohistochemistry revealed that the CR group had significantly higher expression of Bax (P<0.001) and significantly lower levels of Bcl2 (P<0.0001) and Ki67 (P<0.001) compared with control group. Furthermore, data from 16S ribosomal (r)RNA sequencing implied that CR was able to reprogram the microbiota structure, characterized by increased Lactobacillus constituent ratio (P<0.05), with amelioration of microbial dysbiosis caused by CRC. Further receiver operating characteristic curves demonstrated that the bacteria Bacteroides [area under the curve (AUC)=0.800], Lactobacillus (AUC=0.760) and Roseburia (AUC=0.720) served key roles in suppression of CRC in the mouse model. The functional prediction of intestinal flora indicated ‘cyanoamino acid metabolism’ (P<0.01), ‘replication initiation protein REP (rolling circle plasmid replication)’ (P<0.01), ‘tRNA G10 N‑methylase Trm11’ (P<0.01) and ‘uncharacterized protein with cyclophilin fold, contains DUF369 domain’ (P<0.05) were downregulated in CR group. These findings implied that CR suppressed CRC in mice and altered the gut microbiota. AD - Department of Gastroenterology, General Hospital, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China AU - Dai,Xing-Chen AU - Zhang,Yu-Huan AU - Huang,Yong-Li AU - Wu,Xiao-Ting AU - Fang,Yu-Jie AU - Gao,Yu-Jing AU - Wang,Fang DA - 2023/01/01 DO - 10.3892/etm.2022.11758 IS - 1 JO - Exp Ther Med KW - calorie restriction colorectal cancer gut microbiota 16S rRNA BALB/c nude mice PY - 2023 SN - 1792-0981 1792-1015 SP - 59 ST - Calorie restriction remodels gut microbiota and suppresses tumorigenesis of colorectal cancer in mice T2 - Experimental and Therapeutic Medicine TI - Calorie restriction remodels gut microbiota and suppresses tumorigenesis of colorectal cancer in mice UR - https://doi.org/10.3892/etm.2022.11758 VL - 25 ER -