TY - JOUR
AB - Colorectal cancer (CRC) is one of the most common cancers worldwide and the consumption of a high‑calorie diet is one of its risk factors. Calorie restriction (CR) slows tumor growth in a variety of cancers, including colorectal cancer; however, the mechanism behind this remains unknown. In the present study, CR effectively reduced the tumor volume and weight in a xenograft BALB/c male nude mouse model. In addition, tumor immunohistochemistry revealed that the CR group had significantly higher expression of Bax (P<0.001) and significantly lower levels of Bcl2 (P<0.0001) and Ki67 (P<0.001) compared with control group. Furthermore, data from 16S ribosomal (r)RNA sequencing implied that CR was able to reprogram the microbiota structure, characterized by increased Lactobacillus constituent ratio (P<0.05), with amelioration of microbial dysbiosis caused by CRC. Further receiver operating characteristic curves demonstrated that the bacteria Bacteroides [area under the curve (AUC)=0.800], Lactobacillus (AUC=0.760) and Roseburia (AUC=0.720) served key roles in suppression of CRC in the mouse model. The functional prediction of intestinal flora indicated ‘cyanoamino acid metabolism’ (P<0.01), ‘replication initiation protein REP (rolling circle plasmid replication)’ (P<0.01), ‘tRNA G10 N‑methylase Trm11’ (P<0.01) and ‘uncharacterized protein with cyclophilin fold, contains DUF369 domain’ (P<0.05) were downregulated in CR group. These findings implied that CR suppressed CRC in mice and altered the gut microbiota.
AD - Department of Gastroenterology, General Hospital, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
AU - Dai,Xing-Chen
AU - Zhang,Yu-Huan
AU - Huang,Yong-Li
AU - Wu,Xiao-Ting
AU - Fang,Yu-Jie
AU - Gao,Yu-Jing
AU - Wang,Fang
DA - 2023/01/01
DO - 10.3892/etm.2022.11758
IS - 1
JO - Exp Ther Med
KW - calorie restriction
colorectal cancer
gut microbiota
16S rRNA
BALB/c nude mice
PY - 2023
SN - 1792-0981
1792-1015
SP - 59
ST - Calorie restriction remodels gut microbiota and suppresses tumorigenesis of colorectal cancer in mice
T2 - Experimental and Therapeutic Medicine
TI - Calorie restriction remodels gut microbiota and suppresses tumorigenesis of colorectal cancer in mice
UR - https://doi.org/10.3892/etm.2022.11758
VL - 25
ER -