TY - JOUR AB - Mitochondrial transplantation is a popular field of research in cell‑free therapy. Menstrual stem cells (MenSCs) are potential donor cells for provision of foreign mitochondria. The present study aimed to investigate the potential effects of MenSC‑derived mitochondria on ovarian cancer from the perspective of protein expression profiling. MenSCs were harvested from menstrual blood. The mitochondria were isolated from MenSCs and ovarian cancer cell line SKOV3. A label‑free mitochondria proteomics and analysis were performed by comparing the protein expression in mitochondria of MenSCs and SKOV3 cells. The differentially expressed proteins with fold‑change >2 were analyzed by Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway and protein domain enrichment, protein interaction networks and parallel reaction monitoring (PRM) analysis. In total, 592 proteins that were found to have increased expression in the mitochondria of MenSCs were analyzed. Functional enrichment analysis revealed these proteins were enriched in metabolism‑associated pathway entries including ‘oxidative phosphorylation’ (OXPHOS) pathway. PRM analysis confirmed that four of 6 candidate proteins in the OXPHOS pathway showed similar increasing trends. The protein domain enrichment analysis showed that domains such as ‘thioredoxin domain’ were significantly enriched. Based on these findings, it was hypothesized that mitochondria from MenSCs have the potential to enhance progression of ovarian cancer likely mediated by the enrichment of OXPHOS‑associated metabolic pathways. AD - Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China Department of Obstetrics and Gynecology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China AU - Chen,Xiuhui AU - Zhong,Wen AU - Chang,Yue AU - Song,Tiefang AU - Liu,Botong AU - Kong,Xianchao AU - Kong,Qingfei DA - 2023/02/01 DO - 10.3892/etm.2023.11798 IS - 2 JO - Exp Ther Med KW - energy metabolism menstrual stem cell mitochondria transplantation oxidative phosphorylation proteomics PY - 2023 SN - 1792-0981 1792-1015 SP - 99 ST - Comparative proteomic analysis of the mitochondria of menstrual stem cells and ovarian cancer cells T2 - Experimental and Therapeutic Medicine TI - Comparative proteomic analysis of the mitochondria of menstrual stem cells and ovarian cancer cells UR - https://doi.org/10.3892/etm.2023.11798 VL - 25 ER -