TY - JOUR AB - Collagen type IV α1 chain (COL4A1) is a collagen protein that acts as a tumor‑promoting factor in several types of cancer. However, the role and the potential mechanisms involving COL4A1 in oral squamous cell carcinoma (OSCC) remain unclear. Using reverse transcription‑quantitative PCR and western blotting, the expression levels of COL4A1 and (nidogen‑1) NID1 in OSCC cells were assessed. Cell Counting Kit‑8, EdU staining and colony formation assays were used to evaluate cell proliferation. Cell migration and invasion were assessed using wound healing and Transwell invasion assays, respectively. The expression levels of proteins involved in epithelial‑mesenchymal transition (EMT) were assessed using western blotting. In addition, the association between COL4A1 and NID1 was analyzed using TNMplot and the STRING database and verified by co‑immunoprecipitation analysis. COL4A1 expression was found to be significantly increased in OSCC cells. Knockdown of COL4A1 expression decreased SCC‑4 cell proliferation, migration and invasion, as well as the progression of EMT. In addition, COL4A1 was shown to be significantly positively associated with NID1 in OSCC and to bind to NID1. NID1 overexpression reversed the inhibitory effects of COL4A1 knockdown on cell proliferation, migration and invasion as well as on the progression of EMT in OSCC cells. In summary, the present findings demonstrated that COL4A1 promoted cell proliferation and migration as well as the progression of EMT in OSCC cells by binding to NID1, highlighting a potential avenue for therapeutic management of OSCC. AD - The Affiliated Stomatological Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China AU - Tian,Xiaobei AU - Sun,Jian AU - Li,Changshun AU - Zhang,Kun DA - 2023/04/01 DO - 10.3892/etm.2023.11875 IS - 4 JO - Exp Ther Med KW - collagen type IV α1 chain nidogen‑1 oral squamous cell carcinoma epithelial‑mesenchymal transition metastasis PY - 2023 SN - 1792-0981 1792-1015 SP - 176 ST - COL4A1 promotes the proliferation and migration of oral squamous cell carcinoma cells by binding to NID1 T2 - Experimental and Therapeutic Medicine TI - COL4A1 promotes the proliferation and migration of oral squamous cell carcinoma cells by binding to NID1 UR - https://doi.org/10.3892/etm.2023.11875 VL - 25 ER -