TY - JOUR AB - Oxidative stress and inflammation are both involved in the pathogenesis of lung ischemia‑reperfusion (I/R) injury. Sulforaphane (SFN) is a natural product with cytoprotective, anti‑inflammatory, and antioxidant properties. The present study hypothesized that SFN may protect against lung I/R injury via the regulation of antioxidant and anti‑inflammatory‑related pathways. A rat model of lung I/R injury was established, and rats were randomly divided into 3 groups: Sham group, I/R group, and SFN group. It was shown that SFN protected against a pathological inflammatory response via inhibition of neutrophil accumulation and in the reduction of the serum levels of the pro‑inflammatory cytokines, IL‑6, IL‑1β, and TNF‑α. SFN treatment also significantly inhibited lung reactive oxygen species production, decreased the levels of 8‑OH‑dG and malondialdehyde, and reversed the decrease in the antioxidant activities of the enzymes catalase, superoxide dismutase, and glutathione peroxidase in the lungs of the I/R treated rats. In addition, SFN ameliorated I/R‑induced lung apoptosis in rats by suppressing Bax and cleaved caspase‑3 levels and increased Bcl‑2 expression. Furthermore, SFN treatment activated an Nrf2‑related antioxidant pathway, as indicated by the increased nuclear transfer of Nrf2 and the downstream HO‑1 and NADPH quinone oxidoreductase‑1. In conclusion, these findings suggested that SFN protected against I/R‑induced lung lesions in rats via activation of the Nrf2/HO‑1 pathway and the accompanied anti‑inflammatory and anti‑apoptotic effects. AD - Department of Respiratory and Critical Care Medicine, Yantaishan Hospital, Yantai, Shandong 264001, P.R. China Department of Respiratory, Yantai Beihai Hospital, Yantai, Shandong 265701, P.R. China Department of Emergency, Tai'an Central Hospital, Tai'an, Shandong 271000, P.R. China AU - Zhang,Liang AU - Wang,Shuxian AU - Zhang,Ying AU - Li,Fenghuan AU - Yu,Chaoxiao DA - 2023/06/01 DO - 10.3892/etm.2023.11964 IS - 6 JO - Exp Ther Med KW - Sulforaphane lung ischemia‑reperfusion injury Nrf‑2/HO‑1 reactive oxygen species PY - 2023 SN - 1792-0981 1792-1015 SP - 265 ST - Sulforaphane alleviates lung ischemia‑reperfusion injury through activating Nrf‑2/HO‑1 signaling T2 - Experimental and Therapeutic Medicine TI - Sulforaphane alleviates lung ischemia‑reperfusion injury through activating Nrf‑2/HO‑1 signaling UR - https://doi.org/10.3892/etm.2023.11964 VL - 25 ER -