TY - JOUR AB - Serial subcultures of normal human oral keratinocytes (NHOKs) and normal human epidermal keratinocytes (NHEKs) to the postmitotic stage result in terminal differentiation and replicative senescence. In order to investigate the common mode of differentiation and/or senescence between mucosal and epidermal keratinocytes, gene expression profiling on both NHOKs and NHEKs was performed by a cDNA microarray analysis. Primary NHOKs and NHEKs were serially subcultured, and the expression level of 3,063 genes was compared between the exponentially growing and senescent cultures. The senescent NHOKs and NHEKs highly expressed 55 and 37 genes, respectively. Among these genes, 16 genes were common in both NHOKs and NHEKs while the other genes were upregulated either in the NHOKs or in the NHEKs. Furthermore, the expression levels of the common genes did not change in the human diploid fibroblasts during the subcultures. These results suggest that subculture-induced differentiation and/or replicative senescence in NHOKs and NHEKs has similar characteristics, but that the pathways leading to these processes are distinct and keratinocyte specific. AD - Department of Pharmacology and Dental Therapeutics, Dental Research Institute, Seoul, Korea null AU - Baek,Jeong-Hwa AU - Lee,Gene AU - Kim,Si,Nae AU - Kim,Jin-Man AU - Kim,Moonkyu AU - Chung,Sung-Chang AU - Min,Byung-Moo DA - 2003/09/01 DO - 10.3892/ijmm.12.3.319 EP - 325 IS - 3 JO - Int J Mol Med PY - 2003 SN - 1107-3756 1791-244X SP - 319 ST - Common genes responsible for differentiation and senescence of human mucosal and epidermal keratinocytes T2 - International Journal of Molecular Medicine TI - Common genes responsible for differentiation and senescence of human mucosal and epidermal keratinocytes UR - https://doi.org/10.3892/ijmm.12.3.319 VL - 12 ER -