TY - JOUR AB - In the present study, we examined whether Acer okamotoanum (A. okamotoanum) sap decreased the serum alcohol and acetaldehyde levels after acute ethanol treatment in a rat model. Male rats were orally administered 25, 50 or 100% A. okamotoanum sap 30 min prior to oral challenge with 3 ml of ethanol (15 ml/kg of a 20% ethanol solution in water), and the blood concentrations of alcohol and acetaldehyde were analyzed up to 7 h after the treatment. Pre-treatment with the sap significantly decreased the blood ethanol and acetaldehyde concentrations after 5 h when compared with ethanol treatment alone (a negative control). The expression levels of liver alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) mRNA were increased significantly in animals pre-treated with A. okamotoanum sap when compared with negative and positive controls. The data suggest that sap pre-treatment enhanced the alcohol metabolism rate in the rat liver. To investigate the involvement of mitochondrial regulation in the ethanol-induced hepatocyte apoptosis, we carried out an immunohistochemical analysis of Bax and Bcl-2. Pre-treatment with sap significantly decreased Bax expression and increased Bcl-2 expression 7 h after ethanol administration when compared with the negative control. The data suggest that A. okamotoanum sap pre-treatment may reduce the alcohol-induced oxidative stress in the rat liver. AD - Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea null Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea AU - Yoo,Yeong-Min AU - Jung,Eui-Man AU - Kang,Ha-Young AU - Choi,In-Gyu AU - Choi,Kyung-Chul AU - Jeung,Eui-Bae DA - 2011/10/01 DO - 10.3892/ijmm.2011.724 EP - 495 IS - 4 JO - Int J Mol Med KW - Acer okamotoanum acetaldehyde alcohol dehydro­genase aldehyde dehydrogenase PY - 2011 SN - 1107-3756 1791-244X SP - 489 ST - The sap of Acer okamotoanum decreases serum alcohol levels after acute ethanol ingestion in rats T2 - International Journal of Molecular Medicine TI - The sap of Acer okamotoanum decreases serum alcohol levels after acute ethanol ingestion in rats UR - https://doi.org/10.3892/ijmm.2011.724 VL - 28 ER -