TY - JOUR AB - Obesity is a risk factor associated with numerous disorders, such as type 2 diabetes, hypertension, dyslipidemia and coronary heart disease. In this study, we investigated the inhibitory effects of Pericarpium zanthoxyli extract (PZE) on the adipocytic differentiation of OP9 cells. During adipocyte differentiation, the OP9 cells were treated with 0, 10 and 20 µg/ml of PZE at various time intervals, followed by the examination of lipid droplet formation and the mRNA expression of adipogenesis-related genes. The cells treated with PZE during the early period (days 0-2) showed a significant reduction in the accumulation of lipid droplets, which were induced by a standard adipogenic cocktail, as well as a decrease in the expression of the adipogenesis-related transcription factor, peroxisome proliferator-activated receptor γ (PPARγ) and PPARγ-target genes, such as adipocyte protein 2 (aP2), fatty acid synthase (FAS) and other adipocyte markers. Adipocyte differentiation was not inhibited by treatment with PZE during the late stage of differentiation (days 3-5). Thus, the inhibitory effects of PZE on adipocyte differentiation occurred during the early stages of adipogenesis, which was confirmed by the decrease in the levels of CCAAT/enhancer-binding protein β (C/EBPβ) in a dose-dependent manner when the OP9 cells were exposed to PZE. Taken together, our results indicate that PZE inhibit the early stages of adipogenic differentiation by inhibiting C/EBPβ expression. AD - Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea Department of Internal Medicine, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea Department of Family Medicine, The Catholic University of Korea, Incheon St. Mary's Hospital, Bupyeong-gu, Incheon 403-720, Republic of Korea Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea Department of Korean Physiology, Wonkwang University School of Korean Medicine, Iksan, Jeonbuk 570-749, Republic of Korea AU - Kim,Ha-Rim AU - Kim,Jeong-Mi AU - Kim,Mi-Seong AU - Hwang,Jin-Ki AU - Yang,Sei-Hoon AU - Kim,Hye-Jung AU - Lee,Dong-Sung AU - Oh,Hyuncheol AU - Kim,Youn-Chul AU - Ryu,Do-Gon AU - Lee,Young-Rae AU - Kwon,Kang-Beom DA - 2014/05/01 DO - 10.3892/ijmm.2014.1667 EP - 1146 IS - 5 JO - Int J Mol Med KW - Pericarpium zanthoxyli extract differentiation adipocyte peroxisome proliferator-activated receptor γ CCAAT/enhancer-binding protein β PY - 2014 SN - 1107-3756 1791-244X SP - 1140 ST - Inhibitory effects of Pericarpium zanthoxyli extract on adipocyte differentiation T2 - International Journal of Molecular Medicine TI - Inhibitory effects of Pericarpium zanthoxyli extract on adipocyte differentiation UR - https://doi.org/10.3892/ijmm.2014.1667 VL - 33 ER -