TY - JOUR AB - Concentrated growth factor (CGF) is a newly generated complex that comprises a fibrin matrix incorporating growth factors and plasmatic and leukocyte cytokines. It has been widely used in bone regenerative medicine. However, the effect of CGF on peripheral nerve regeneration had not been previously investigated. The aim of the present study was to evaluate the possibility of using CGF for nerve regeneration by i) investigating the effect of CGF on the proliferation of Schwann cells (SCs) and secretion of neurotrophic factors nerve growth factor (NGF) and glial cell line‑derived neurotrophic factor (GDNF) in vitro; and ii) analyzing the effect of CGF on functional nerve recovery after nerve injury in vivo. CGF was prepared from venous blood taken from rats, and using scanning electron microscopy (SEM) we noted that it featured a fiber‑like appearance with pore size ranging from 0.1 to 1.0 µm. The soluble component of CGF was used to produce conditioned media with which to treat the Schwann cell line. A cell counting kit-8 assay and cell cycle analysis were both used to study the proliferative effect of CGF on SCs. Reverse transcription-quantitative PCR and western blot analysis demonstrated that there was an increase in the mRNA and protein expression of NGF and GDNF, both of which are markers of SC neurotrophic secretion. A model of sciatic nerve crush injury was established for the in vivo experiment, and CGF was found to increase the sciatic functional index (indicative of nerve function). We noted that CGF increased SC proliferation and secretion of neurotrophic factors in vitro, and promoted functional recovery after peripheral nerve injuries in vivo. These results suggest that CGF is a promising candidate biomaterial for peripheral nerve regeneration, and may potentially be utilized to repair nerve injuries. AD - Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China Department of Very Important People, School and Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China Department of Bioengineering, School of Dentistry, The University of Sydney, NSW 2006, Australia AU - Qin,Jie AU - Wang,Lin AU - Sun,Yue AU - Sun,Xiaolin AU - Wen,Chaoju AU - Shahmoradi,Mahdi AU - Zhou,Yanmin DA - 2016/02/01 DO - 10.3892/ijmm.2015.2438 EP - 500 IS - 2 JO - Int J Mol Med KW - concentrated growth factor nerve regeneration Schwann cells proliferation secretion PY - 2016 SN - 1107-3756 1791-244X SP - 493 ST - Concentrated growth factor increases Schwann cell proliferation and neurotrophic factor secretion and promotes functional nerve recovery in vivo T2 - International Journal of Molecular Medicine TI - Concentrated growth factor increases Schwann cell proliferation and neurotrophic factor secretion and promotes functional nerve recovery in vivo UR - https://doi.org/10.3892/ijmm.2015.2438 VL - 37 ER -