TY - JOUR AB - Freshwater animal proteins have long been used as nutrient supplements. In this study, melanian snail (Semisulcospira libertina) protein hydrolysates (MPh) were found to exert anti-diabetic and protective effects against liver and kidney damage in mice with type II diabetes adapted to a 45% kcal high-fat diet (HFD). The hypoglycemic, hepatoprotective and nephroprotective effects of MPh were analyzed after 12 weeks of the continuous oral administration of MPh at 125, 250 and 500 mg/kg. Diabetic control mice exhibited an increase in body weight, and blood glucose and insulin levels, with a decrease in serum high-density lipoprotein (HDL) levels. In addition, an increase in the regions of steatohepatitis, hepatocyte hypertrophy, and lipid droplet deposit-related renal tubular vacuolation degenerative lesions were detected, with noticeable expansion and hyperplasia of the pancreatic islets, and an increase in glucagon- and insulin-producing cells, insulin/glucagon cell ratios in the endocrine pancreas and hepatic lipid peroxidation, as well as decreased zymogen contents. Furthermore, a deterioration of the endogenous antioxidant defense system was observed, with reduced glucose utilization related hepatic glucokinase (GK) activity and an increase in hepatic gluconeogenesis-related phosphoenolpyruvate carboxykinase (PEPCK) and glucose‑6-phosphatase (G6pase) activity. However, all of these diabetic complications were significantly inhibited by oral treatment with MPh in a dose-dependent manner. In addition, the marked dose-dependent inhibition of hepatic lipid peroxidation, the depletion of the liver endogenous antioxidant defense system, and changes in hepatic glucose-regulating enzyme activities were also observed. The results of this study suggest that MPh exerts potent anti-diabetic effects, along with the amelioration of related complications in mice with type II diabetes. The overall effects of MPh at a dose of 125 mg/kg on HFD-induced diabetes and related complications were similar or more potent than those of metformin (250 mg/kg). AD - Major in Food Biotechnology, Division of Bioindustry, College of Medical and Life Sciences, Silla University, Sasang-gu, Busan 46958, Republic of Korea Aribio Inc., Byeoksan Digital Valley, Yeongdeungpo-gu, Seoul 07286, Republic of Korea Department of Biotechnology, College of Fisheries Science, Pukyong National University, Nam-Gu, Busan 48513, Republic of Korea Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University, Gyeongsan-si, Gyeongsangbuk-do 38610, Republic of Korea AU - Choi,Jae-Suk AU - Kim,Joo-Wan AU - Park,Jeong ,Been AU - Pyo,Sang ,Eun AU - Hong,Yong-Ki AU - Ku,Sae ,Kwang AU - Kim,Mi-Ryung DA - 2017/06/01 DO - 10.3892/ijmm.2017.2967 EP - 1451 IS - 6 JO - Int J Mol Med KW - melanian snail Semisulcospira libertina protein hydrolysates blood glycemia-modulating effects anti-diabetic effects PY - 2017 SN - 1107-3756 1791-244X SP - 1437 ST - Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes T2 - International Journal of Molecular Medicine TI - Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes UR - https://doi.org/10.3892/ijmm.2017.2967 VL - 39 ER -