TY - JOUR AB - Neuro-oncological ventral antigen 1 (Nova1) is a well known brain-specific splicing factor. Several studies have identified Nova1 as a regulatory protein at the top of a hierarchical network. However, the function of Nova1 during hypoxia remains poorly understood. This study aimed to investigate the protective effect of Nova1 against cell hypoxia and to further explore the Bax/Bcl-2/caspase-3 pathway as a potential mechanism. During hypoxia, the survival rate of pheochromocytoma PC12 cells was gradually decreased and the apoptosis rate was gradually increased, peaking at 48 h of hypoxia. At 48 h after transfection of PC12 cells with pCMV-Myc-Nova1, the expression of Nova1 was significantly increased, with wide distribution in the cytoplasm and nucleus. Moreover, the survival rate was significantly increased and the apoptosis rate was significantly decreased. Additionally, the mRNA and protein expression levels of Bax and caspase-3 were significantly increased in the pCMV-Myc group and significantly decreased in the pCMV‑Myc-Nova1 group, whereas that of Bcl-2 was significantly decreased in the pCMV-Myc group and significantly increased in the pCMV-Myc-Nova1 group. This study indicated that Nova1 could be linked to resistance to the hypoxia-induced apoptosis of PC12 cells via the Bax/Bcl-2/caspase-3 pathway, and this finding may be of significance for exploring novel mechanisms of hypoxia and the treatment of hypoxia-associated diseases. AD - Department of Biochemistry, Medical College of Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China Department of Biochemistry, Biosciences and Biotechnology College of Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, Jiangsu 225001, P.R. China AU - Li,Hualing AU - Lv,Bei AU - Kong,Ling AU - Xia,Jing AU - Zhu,Ming AU - Hu,Lijuan AU - Zhen,Danyang AU - Wu,Yifan AU - Jia,Xiaoqin AU - Zhu,Sujuan AU - Cui,Hengmi DA - 2017/10/01 DO - 10.3892/ijmm.2017.3089 EP - 1133 IS - 4 JO - Int J Mol Med KW - neuro-oncological ventral antigen 1 resistance hypoxia apoptosis pathway PY - 2017 SN - 1107-3756 1791-244X SP - 1125 ST - Nova1 mediates resistance of rat pheochromocytoma cells to hypoxia-induced apoptosis via the Bax/Bcl-2/caspase-3 pathway T2 - International Journal of Molecular Medicine TI - Nova1 mediates resistance of rat pheochromocytoma cells to hypoxia-induced apoptosis via the Bax/Bcl-2/caspase-3 pathway UR - https://doi.org/10.3892/ijmm.2017.3089 VL - 40 ER -