TY - JOUR AB - The PC12 cell line is a classical neuronal cell model due to its ability to acquire the sympathetic neurons features when deal with nerve growth factor (NGF). In the present study, the authors used a variety of different methods to induce PC12 cells, such as Opti-MEM medium containing different concentrations of fetal bovine serum (FBS) and horse serum compared with RPMI-1640 medium, and then observed the neurite length, differentiation, adhesion, cell proliferation and action potential, as well as the protein levels of axonal growth-associated protein 43 (GAP-43) and synaptic protein synapsin-1, among other differences. Compared with the conventional RPMI-1640 medium induction method, the new approach significantly improved the neurite length of induced cells (2.7 times longer), differentiation rate (30% increase), adhesion rate (21% increase) and expression of GAP-43 and synapsin-1 (three times), as well as reduced cell proliferation. The morphology of induced cells in Opti-MEM medium containing 0.5% FBS was more like that of neurons. Additionally, induced cells were also able to motivate the action potential after treatment for 6 days. Therefore, the research provided a novel, improved induction method of neural differentiation of PC12 cells using Opti-MEM medium containing 0.5% FBS, resulting in a better neuronal model cell line that can be widely used in neurobiology and neuropharmacology research. AD - Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, Guangdong 510632, P.R. China College of Pharmacy, Jinan University, Guangzhou, Guangdong 510632, P.R. China Department of Anesthesia and Intensive Care, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong 999077, SAR, P.R. China Department of Ophthalmology, The First Clinical Medical College of Jinan University, Guangzhou, Guangdong 510632, P.R. China AU - Hu,Rendong AU - Cao,Qiaoyu AU - Sun,Zhongqing AU - Chen,Jinying AU - Zheng,Qing AU - Xiao,Fei DA - 2018/01/01 DO - 10.3892/ijmm.2017.3195 EP - 201 IS - 1 JO - Int J Mol Med KW - PC12 cells neural differentiation Opti-MEM induced method PY - 2018 SN - 1107-3756 1791-244X SP - 195 ST - A novel method of neural differentiation of PC12 cells by using Opti-MEM as a basic induction medium T2 - International Journal of Molecular Medicine TI - A novel method of neural differentiation of PC12 cells by using Opti-MEM as a basic induction medium UR - https://doi.org/10.3892/ijmm.2017.3195 VL - 41 ER -