TY - JOUR AB - Although genome-wide association studies have shown that potassium voltage-gated channel subfamily Q member 1 (KCNQ1) is one of the genes that is most significantly associated with type 2 diabetes mellitus (T2DM), functionally annotating disease-associated single nucleotide polymorphisms (SNPs) remains a challenge. Recently, our group described a novel strategy to identify proteins that bind to SNP-containing loci in an allele-specific manner. The present study successfully applied this strategy to investigate rs163184, a T2DM susceptibility SNP located in the intronic region of KCNQ1. Comparative analysis of DNA-binding proteins revealed that the binding activities for the genomic region containing SNP rs163184 differed between alleles for several proteins, including Sp3 and Lsd1/Kdm1a. Sp3 preferentially bound to the non-risk rs163184 allele and stimulated transcriptional activity in an artificial promoter containing this region. Lsd1/Kdm1a was identified to be preferentially recruited to the non-risk allele of the rs163184 region and reduced Sp3-dependent transcriptional activity in the artificial promoter. In addition, expression of the nearby cyclin‑dependent kinase inhibitor 1C (CDKN1C) gene was revealed to be upregulated after SP3 knockdown in cells that possessed non-risk alleles. This suggests that CDKN1C is potentially one of the functional targets of SNP rs163184, which modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a. AD - Department of Metabolic Disorder, Diabetes Research Center, National Center for Global Health and Medicine, Tokyo 162-8655, Japan Department of Diabetic Complications, Diabetes Research Center, National Center for Global Health and Medicine, Tokyo 162-8655, Japan Department of Biochemistry, Tokyo Medical University, Tokyo 160-8402, Japan AU - Hiramoto,Masaki AU - Udagawa,Haruhide AU - Ishibashi,Naoko AU - Takahashi,Eri AU - Kaburagi,Yasushi AU - Miyazawa,Keisuke AU - Funahashi,Nobuaki AU - Nammo,Takao AU - Yasuda,Kazuki DA - 2018/02/01 DO - 10.3892/ijmm.2017.3273 EP - 728 IS - 2 JO - Int J Mol Med KW - type 2 diabetes potassium voltage-gated channel subfamily Q member 1 single nucleotide polymorphism DNA-binding proteins cyclin‑dependent kinase inhibitor 1C PY - 2018 SN - 1107-3756 1791-244X SP - 717 ST - A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression T2 - International Journal of Molecular Medicine TI - A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression UR - https://doi.org/10.3892/ijmm.2017.3273 VL - 41 ER -