TY - JOUR AB - Breast cancer-specific gene 1 (BCSG1), also referred to as γ-synuclein (SNCG), is highly expressed in human infiltrating breast carcinomas, but not in normal or benign breast tissue. The present study aimed to evaluate the effects of BCSG1 siRNA delivered by lentiviral vector on breast cancer cells and investigate the underlying mechanisms. BCSG1 RNAi lentiviral vector was constructed and transfected into MDA-MB-231 cells. BCSG1 mRNA levels were determined by quantitative polymerase chain reaction analysis. Cell proliferation, migration and apoptosis were evaluated by using the cell counting kit‑8, Transwell assay and flow cytometry, respectively, followed by western blotting to determine the relative levels of AKT, extracellular signal‑regulated kinase (ERK), p-AKT and p-ERK expression. BCSG1 mRNA levels were significantly reduced in MDA-MB‑231 cells following transfection of BCSG1 siRNA delivered by lentiviral vector. Cell migration and proliferation were significantly decreased and the cell cycle was arrested. Western blot analysis indicated that the protein levels of p-AKT and p-ERK were significantly lower in the BCSG1 siRNA-treated groups compared with the control and negative control groups. Therefore, BCSG1 siRNA delivered by lentiviral vector was able to significantly reduce BCSG1 expression, suppress cell migration and proliferation, possibly through the reduction of the protein levels of p-AKT and p-ERK. AD - Department of Breast Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518035, P.R. China Biobank, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shenzhen, Shenzhen, Guangdong 518035, P.R. China Department of Laboratory Medicine and Pathology, Masonic Cancer Center, University of Minnesota, MN Twin Cities, MN 55455, USA Department of Pathology, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shenzhen, Shenzhen, Guangdong 518035, P.R. China Department of Breast Surgery, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shenzhen, Shenzhen, Guangdong 518035, P.R. China Clinical Research Centre, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China Cancer Research Centre, School of Medicine, Shenzhen University, Shenzhen, Guangdong 518060, P.R. China AU - He,Jin-Song AU - Xie,Ni AU - Yang,Jian-Bo AU - Guan,Hong AU - Chen,Wei-Cai AU - Zou,Chang AU - Ouyang,Yi-Wen AU - Mao,You-Sheng AU - Luo,Xue-Ying AU - Pan,Yue AU - Fu,Li DA - 2018/03/01 DO - 10.3892/ijmm.2017.3355 EP - 1664 IS - 3 JO - Int J Mol Med KW - breast cancer breast cancer-specific gene 1 small interfering RNA lentiviral vector PY - 2018 SN - 1107-3756 1791-244X SP - 1659 ST - BCSG1 siRNA delivered by lentiviral vector suppressed proliferation and migration of MDA-MB-231 cells T2 - International Journal of Molecular Medicine TI - BCSG1 siRNA delivered by lentiviral vector suppressed proliferation and migration of MDA-MB-231 cells UR - https://doi.org/10.3892/ijmm.2017.3355 VL - 41 ER -