TY - JOUR AB - In the present study, the neuroprotective potential of magnolol against ischemia-reperfusion brain injury was examined via in vivo and in vitro experiments. Magnolol exhibited strong radical scavenging and antioxidant activity, and significantly inhibited the production of interleukin‑6, tumor necrosis factor‑a and nitrite/nitrate (NOX) in lipopolysaccharide-stimulated BV2 and RAW 264.7 cells when applied at concentrations of 10 and 50 µM, respectively. Magnolol (100 µM) also significantly attenuated oxygen‑glucose deprivation‑induced damage in neonatal rat hippocampal slice cultures, when administered up to 4 h following the insult. In a rat model of stable ischemia, compared with a vehicle‑treated ischemic control, pretreatment with magnolol (0.01‑1 mg/kg, intravenously) significantly reduced brain infarction following ischemic stroke, and post‑treatment with magnolol (1 mg/kg) remained effective and significantly reduced infarction when administered 2 h following the onset of ischemia. Additionally, magnolol (0.3 and 1 mg/kg) significantly reduced the accumulation of superoxide anions at the border zones of infarction and reduced oxidative damage in the ischemic brain. This was assessed by measuring the levels of NOX, malondialdehyde and myeloperoxidase, the ratio of glutathione/oxidized glutathione and the immunoreactions of 8‑hydroxy‑2'‑deoxyguanosine and 4‑hydroxynonenal. Thus, magnolol was revealed to protect against ischemia‑reperfusion brain damage. This may be partly attributed to its antioxidant, radical scavenging and anti‑inflammatory effects. AD - Institute of Biomedical Engineering, National Cheng Kung University, Tainan 70428, Taiwan, R.O.C. Neurophysiology Laboratory, Department of Surgery, National Cheng Kung University Medical Center and Medical School, Tainan 70428, Taiwan, R.O.C. AU - Huang,Sheng‑Yang AU - Tai,Shih‑Huang AU - Chang,Che‑Chao AU - Tu,Yi‑Fang AU - Chang,Chih‑Han AU - Lee,E‑Jian DA - 2018/04/01 DO - 10.3892/ijmm.2018.3387 EP - 2262 IS - 4 JO - Int J Mol Med KW - ischemic stroke focal cerebral ischemia oxygen-glucose deprivation neuroprotection magnolol superoxide and oxidative damage PY - 2018 SN - 1107-3756 1791-244X SP - 2252 ST - Magnolol protects against ischemic-reperfusion brain damage following oxygen-glucose deprivation and transient focal cerebral ischemia T2 - International Journal of Molecular Medicine TI - Magnolol protects against ischemic-reperfusion brain damage following oxygen-glucose deprivation and transient focal cerebral ischemia UR - https://doi.org/10.3892/ijmm.2018.3387 VL - 41 ER -