TY - JOUR AB - Intestinal ischemia and reperfusion (II/R) injury often triggers severe injury in remote organs, with the lungs being considered the main target. Excessive elevation of proinflammatory cytokines is a major contributor in the occurrence and development of II/R‑induced acute lung injury (ALI). Therefore, the present study aimed to investigate whether blocking tumor necrosis factor‑α (TNF‑α) expression could protect the lungs from injury following II/R, and to explore the possible underlying mechanism involving interleukin‑10 (IL‑10). Briefly, II/R was induced in rats by 40 min occlusion of the superior mesenteric artery and celiac artery, followed by 8, 16 or 24 h of reperfusion. Subsequently, lentiviral vectors containing TNF‑α short hairpin (sh)RNA were injected into the right lung tissues, in order to induce TNF‑α knockdown. The severity of ALI was determined according to lung injury scores and lung edema (lung wet/dry weight ratio). The expression levels of TNF‑α were analyzed by quantitative polymerase chain reaction (qPCR), western blotting and immunofluorescence (IF) staining. IL‑10 expression, in response to TNF‑α knockdown, was detected in lung tissues by qPCR and IF. The results detected marked inflammatory responses, and increased levels of lung wet/dry weight ratio and TNF‑α expression, in the lungs of II/R rats. Conversely, treatment with TNF‑α shRNA significantly alleviated the severity of ALI and upregulated the expression levels of IL‑10 in lung tissues. These findings suggested that TNF‑α RNA interference may exert a protective effect on II/R‑induced ALI via the upregulation of IL‑10. Therefore, TNF‑α knockdown may be considered a potential strategy for the prevention or treatment of ALI induced by II/R in future clinical trials. AD - Department of Respiration, First People's Hospital of Yunnan Province, Kunming, Yunnan 650032, P.R. China Department of Anesthesiology, Sun Yat‑Sen Memorial Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China Department of Anesthesiology and Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China Institute of Neuroscience, Kunming Medical University, Kunming, Yunnan 650500, P.R. China AU - Yang,Zhen AU - Zhang,Xue‑Rong AU - Zhao,Qiong AU - Wang,Sheng‑Lan AU - Xiong,Liu‑Lin AU - Zhang,Piao AU - Yuan,Bing AU - Zhang,Zi‑Bing AU - Fan,Shu‑Yuan AU - Wang,Ting‑Hua AU - Zhang,Yun‑Hui DA - 2018/08/01 DO - 10.3892/ijmm.2018.3674 EP - 934 IS - 2 JO - Int J Mol Med KW - tumor necrosis factor‑α interleukin‑10 RNA interference intestinal ischemia and reperfusion acute lung injury PY - 2018 SN - 1107-3756 1791-244X SP - 926 ST - Knockdown of TNF‑α alleviates acute lung injury in rats with intestinal ischemia and reperfusion injury by upregulating IL‑10 expression T2 - International Journal of Molecular Medicine TI - Knockdown of TNF‑α alleviates acute lung injury in rats with intestinal ischemia and reperfusion injury by upregulating IL‑10 expression UR - https://doi.org/10.3892/ijmm.2018.3674 VL - 42 ER -