TY - JOUR AB - Emerging studies have indicated that the dysregulation of microRNAs (miRNAs or miRs) plays a vital role in the development and metastasis of tumors. However, the role of miR‑93‑5p in esophageal carcinoma (EC) has not been extensively reported. The present study thus focused on the role of miR‑93‑5p and its downstream target in the occurrence and development of EC. Firstly, miRNA expression profiles associated with EC were accessed from the TCGA_ESCA dataset and analyzed. Subsequently, the expression patterns of miR‑93‑5p and TGFβR2 were characterized in the human esophageal cell line, Het‑1A, and the human EC cell lines, TE‑1, Eca‑109 and EC9706, by RT‑qPCR and western blot analysis. WST‑1 assay, flow cytometry, Transwell assay, wound healing assay and bioinformatics analysis were used to explore their functions in EC cells. Finally, a dual‑luciferase reporter assay was employed to determine the targeted association between miR‑93‑5p and TGFβR2. The results revealed that the expression of miR‑93‑5p was markedly higher in EC cell lines compared with that in the normal cell line. The overexpression of miR‑93‑5p facilitated cell proliferation, migration and invasion, and inhibited cell apoptosis. Additionally, TGFβR2 was identified as a functional target of miR‑93‑5p in EC cells, as judged by a series of in vitro experiments. Furthermore, it was found that the simultaneous overexpression of miR‑93‑5p and TGFβR2 almost had no effect on the biological behaviors of EC cells. On the whole, the present study demonstrates that miR‑93‑5p promotes the proliferation, migration and invasion, and inhibits the apoptosis of EC cells by targeting TGFβR2. AD - Department of Thoracic Surgery, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou, Fujian 350014, P.R. China Department of Radiotherapy, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou, Fujian 350014, P.R. China Department of Chest Radiotherapy, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou, Fujian 350014, P.R. China Department of Pathology, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou, Fujian 350014, P.R. China AU - Cai,Yibin AU - Ruan,Weizhong AU - Ding,Jianming AU - Wei,Ning AU - Wang,Jianchao AU - Zhang,Hong AU - Ma,Ning AU - Weng,Guibin AU - Su,Wei,Kun AU - Lin,Yijin AU - Zhu,Kunshou DA - 2021/03/01 DO - 10.3892/ijmm.2020.4836 IS - 3 JO - Int J Mol Med KW - miR‑93‑5p transforming growth factor‑β receptor 2 esophageal carcinoma proliferation migration and invasion apoptosis PY - 2021 SN - 1107-3756 1791-244X SP - 3 ST - miR‑93‑5p regulates the occurrence and development of esophageal carcinoma epithelial cells by targeting TGFβR2 T2 - International Journal of Molecular Medicine TI - miR‑93‑5p regulates the occurrence and development of esophageal carcinoma epithelial cells by targeting TGFβR2 UR - https://doi.org/10.3892/ijmm.2020.4836 VL - 47 ER -