TY - JOUR AB - Mitochondrial abnormalities are primarily seen in morphology, structure and function. They can cause damage to organs, including the heart, brain and muscle, by various mechanisms, such as oxidative stress, abnormal energy metabolism, or genetic mutations. Identifying and detecting pathophysiological alterations in mitochondria is the principal means of studying mitochondrial abnormalities. The present study reviewed methods in mitochondrial research and focused on three aspects: Mitochondrial extraction and purification, morphology and structure and function. In addition to classical methods, such as electron microscopy and mitochondrial membrane potential monitoring, newly developed methods, such as mitochondrial ultrastructural determination, mtDNA mutation assays, metabolomics and analyses of regulatory mechanisms, have also been utilized in recent years. These approaches enable the accurate detection of mitochondrial abnormalities and provide guidance for the diagnosis and treatment of related diseases. AD - Department of Emergency Medicine, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China AU - Yin,Yiyuan AU - Shen,Haitao DA - 2022/10/01 DO - 10.3892/ijmm.2022.5182 IS - 4 JO - Int J Mol Med KW - mitochondria mitochondrial morphology mitochondrial dysfunction mitochondrial DNA mitochondrial diseases PY - 2022 SN - 1107-3756 1791-244X SP - 126 ST - Common methods in mitochondrial research (Review) T2 - International Journal of Molecular Medicine TI - Common methods in mitochondrial research (Review) UR - https://doi.org/10.3892/ijmm.2022.5182 VL - 50 ER -