TY - JOUR AB - Aging is considered a high risk factor for Alzheimer's disease (AD), which is one of the most prevalent neurodegenerative disorders in the elderly population. The major pathologic feature of AD is senile plaques mainly containing amyloid-β (Aβ) components. However, little direct evidence has shown aging in association with Aβ. Here we show that the protein-protein interaction of amyloid precursor protein (APP) and β -site amyloid cleavage enzyme 1 (BACE1) is enhanced by the fluorescence resonance energy transfer (FRET) assay during the aging process, and the APP-BACE1 complex accumulates in the endosome in the IMR-90 fibroblast (NHF) cellular aging models. Moreover, enhanced Aβ is observed in aged cells, rat brain homogenates and human serum. Interestingly, addition of the dominant-negative mutant of Rab5, a small G-protein Rab5 involved in the endocytic process, inhibits the aging-related APP-BACE1 interaction and Aβ production, suggesting that endocytosis contributes to AD progression. AD - Center for Clinical Molecular Medicine, Children's Hospital, Chongqing Medical University, Chongqing 400014, P.R. China. zoulin_74@yahoo.com.cn null AU - Zou,Lin AU - Yang,Rongxi AU - Zhang,Penghui AU - Dai,Yi DA - 2010/03/01 DO - 10.3892/ijmm_00000358 EP - 407 IS - 3 JO - Int J Mol Med PY - 2010 SN - 1107-3756 1791-244X SP - 401 ST - The enhancement of amyloid precursor protein and β-site amyloid cleavage enzyme 1 interaction: Amyloid-β production with aging T2 - International Journal of Molecular Medicine TI - The enhancement of amyloid precursor protein and β-site amyloid cleavage enzyme 1 interaction: Amyloid-β production with aging UR - https://doi.org/10.3892/ijmm_00000358 VL - 25 ER -