TY - JOUR AB - Vascular endothelial growth factor (VEGF) and its receptors are involved in carcinogenesis, invasion and tumor angiogenesis, but the underlying mechanism by which VEGF promotes tumor metastasis is poorly understood. In this study, we show that in cancer patients high expression of VEGF is correlated with metastasis, and anti-VEGF treatment (bevacizumab) has clinical effects on tumor metastasis. Two human lung carcinoma cell lines (A549 and SPCA1 cells) with distinct VEGF expression were injected intravenously through the lateral tail vein of SCID mice and a murine model was developed. We investigated the association between the expression of VEGF and tumor metastasis by microvessel density, immunohistochemistry and whole mount staining. At sacrifice, in the high VEGF expression A549 cell line group, the induced tumor was distinctively larger in size and multiple metastatic lesions were found in lung tissues. Two specific neutralizing anti-mouse VEGFR1 and VEGFR2 antibodies were administered to the tumor-bearing mice; anti-VEGFR1, but not anti-VEGFR2 treatment produced inhibitive effects on VEGF-induced tumor metastasis. These findings demonstrate that the VEGF-VEGFR1 signaling pathway is crucial for tumor metastasis and the blockade of VEGF-VEGFR1-induced metastasis may provide a novel approach for the prevention and treatment of tumor metastasis. AD - Department of Immunology, Shandong Qianfoshan Hospital, Shandong University, 16766 Jingshi Road, Jinan 250014, P.R. China null Cancer Therapy Center, Shandong Provincial Hospital, Shandong University, 324 Jingwuweiqi Road, 250021 Jinan, P.R. China AU - Liu,Wenbo AU - Xu,Jiying AU - Wang,Mingming AU - Wang,Qiangxiu AU - Bi,Yuli AU - Han,Mingyong DA - 2011/11/01 DO - 10.3892/ijo.2011.1138 EP - 1220 IS - 5 JO - Int J Oncol KW - vascular endothelial growth factor metastasis signal pathway VEGFR PY - 2011 SN - 1019-6439 1791-2423 SP - 1213 ST - Tumor-derived vascular endothelial growth factor (VEGF)-A facilitates tumor metastasis through the VEGF-VEGFR1 signaling pathway T2 - International Journal of Oncology TI - Tumor-derived vascular endothelial growth factor (VEGF)-A facilitates tumor metastasis through the VEGF-VEGFR1 signaling pathway UR - https://doi.org/10.3892/ijo.2011.1138 VL - 39 ER -