TY - JOUR AB - Three distinct classes of CpG-oligonucleotides (ODN) (CpG-A, CpG-B and CpG-C) have been identified on the basis of differences in their structures and immune effects. To date, only CpG-B is applied for clinical treatments; however, it is still unknown which of the different CpG-ODN classes is most useful as an adjuvant for human cancer vaccine therapy. In the present study, we examined the activity of these 3 types of CpG-ODN in enhancing the induction of human peptide-specific CTLs. Our data showed that the specific cytotoxicity was augmented in the presence of CpG-A, -B and -C but not in the presence of control ODN, and the augmenting effect was most potent with CpG-A. Flow cytometric analysis showed the subpopulation of effector-memory cells in CD8+ cells was most increased with CpG-A. Furthermore, depletion of PDCs from PBMCs before stimulation with peptide and CpG-ODN completely abrogated the augmenting effect of CpG-ODN. These data indicated that the stimulation of PDCs by CpG-ODN augmented the generation of peptide-specific CTLs, and CpG-A was superior to CpG-B and CpG-C in terms of augmenting the generation of human peptide-specific CTLs in vitro. AD - Second Department of Surgery, School of Medicine, Wakayama Medical University, Wakayama, Japan Second Department of Surgery, School of Medicine, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-8510, Japan null AU - Katsuda,Masahiro AU - Iwahashi,Makoto AU - Matsuda,Kenji AU - Miyazawa,Motoki AU - Nakamori,Mikihito AU - Nakamura,Masaki AU - Ojima,Toshiyasu AU - Iida,Takeshi AU - Hayata,Keiji AU - Yamaue,Hiroki DA - 2011/11/01 DO - 10.3892/ijo.2011.1146 EP - 1302 IS - 5 JO - Int J Oncol KW - CpG-ODN peptide vaccine effector-memory cells PY - 2011 SN - 1019-6439 1791-2423 SP - 1295 ST - Comparison of different classes of CpG-ODN in augmenting the generation of human epitope peptide-specific CTLs T2 - International Journal of Oncology TI - Comparison of different classes of CpG-ODN in augmenting the generation of human epitope peptide-specific CTLs UR - https://doi.org/10.3892/ijo.2011.1146 VL - 39 ER -