TY - JOUR AB - This study examined the role of the immuno­suppressive enzyme indoleamine-2,3-dioxygenase (IDO) in ovarian cancer progression, and the possible application of this enzyme as a target for ovarian cancer therapy. We transfected a short hairpin RNA vector targeting IDO into the human ovarian cancer cell line SKOV-3, that constitutively expresses IDO and established an IDO downregulated cell line (SKOV-3/shIDO) to determine whether inhibition of IDO mediates the progression of ovarian cancer. IDO downregulation suppressed tumor growth and peritoneal dissemination in vivo, without influencing cancer cell growth. Moreover, IDO downregulation enhanced the sensitivity of cancer cells to natural killer (NK) cells in vitro, and promoted NK cell accumulation in the tumor stroma in vivo. These findings indicate that downregulation of IDO controls ovarian cancer progression by activating NK cells, suggesting IDO targeting as a potential therapy for ovarian cancer. AD - Department of Obstetrics and Gynecology, School of Medicine, Jichi Medical University, Tochigi, Japan Department of Obstetrics and Gynecology, School of Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan null AU - Wang,Dongdong AU - Saga,Yasushi AU - Mizukami,Hiroaki AU - Sato,Naoto AU - Nonaka,Hiroaki AU - Fujiwara,Hiroyuki AU - Takei,Yuji AU - Machida,Shizuo AU - Takikawa,Osamu AU - Ozawa,Keiya AU - Suzuki,Mitsuaki DA - 2012/04/01 DO - 10.3892/ijo.2011.1295 EP - 934 IS - 4 JO - Int J Oncol KW - ovarian cancer indoleamine-2 3-dioxygenase peritoneal dissemination natural killer cell short hairpin RNA PY - 2012 SN - 1019-6439 1791-2423 SP - 929 ST - Indoleamine-2,3-dioxygenase, an immunosuppressive enzyme that inhibits natural killer cell function, as a useful target for ovarian cancer therapy T2 - International Journal of Oncology TI - Indoleamine-2,3-dioxygenase, an immunosuppressive enzyme that inhibits natural killer cell function, as a useful target for ovarian cancer therapy UR - https://doi.org/10.3892/ijo.2011.1295 VL - 40 ER -