TY - JOUR AB - Recent reports have demonstrated that another strand of mature microRNA (miRNA), called microRNA* or 3p (5p) strand, which is generated from the same precursor miRNA (Pre-miR), has a crucial role in cellular function. We previously reported the tumor suppressive effect of miR-125a-5p in gastric cancer. The current study was designed to examine the function and clinical significance of miR-125a-3p, a partner strand of miR-125a-5p, in human gastric cancer. Quantitative RT-PCR was used to evaluate miR-125a-3p expression in 70 gastric cancer cases to determine the clinicopathologic significance of miR-125a-3p expression. In addition, the effect of miR-125a-3p on the proliferation of gastric cancer cells was investigated. Low expression levels of miR-125a-3p were associated with indicators of enhanced malignant potential such as tumor size (p=0.0002), tumor invasion (p=0.0149), lymph node metastasis (p=0.018), liver metastasis (p=0.016), peritoneal dissemination (p=0.03), advanced clinical stage (p=0.0037) and poor prognosis (p=0.0083). Multivariate analysis indicated that low miR-125a-3p expression was an independent prognostic factor for survival, while in vitro assays demonstrated that miR‑125a-3p suppressed the proliferation of gastric cancer cells. MiR-125a-3p is a potent prognostic marker in gastric cancer. The clinical significance and tumor suppressive effect of miR‑125a-3p, as well as previously reported miR-125a-5p, suggest that the functional role of another strand of the mature form miRNA cannot be ignored, at least in miR-125a biogenesis. AD - Department of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan Department of Surgery and Molecular Oncology, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumibaru, Beppu, Oita 874-0838, Japan Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan AU - Hashiguchi,Yojiro AU - Nishida,Naohiro AU - Mimori,Koshi AU - Sudo,Tomoya AU - Tanaka,Fumiaki AU - Shibata,Kohei AU - Ishii,Hideshi AU - Mochizuki,Hidetaka AU - Hase,Kazuo AU - Doki,Yuichiro AU - Mori,Masaki DA - 2012/05/01 DO - 10.3892/ijo.2012.1363 EP - 1482 IS - 5 JO - Int J Oncol KW - microRNA miR-125a-3p partner strand clinical significance PY - 2012 SN - 1019-6439 1791-2423 SP - 1477 ST - Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance T2 - International Journal of Oncology TI - Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance UR - https://doi.org/10.3892/ijo.2012.1363 VL - 40 ER -