TY - JOUR AB - Estrogen receptor α (ERα) has been reported to play a critical role in promoting the growth of breast tumor cells. In the present study, we explored the effect of Huaier extract on estrogen receptor α signaling in breast cancer cell lines. Our data demonstrated that Huaier extract effectively inhibited the proliferation of the MCF-7, T47D and ZR-75-1 human breast cancer cell lines. For the mechanism analysis, we demonstrated that Huaier extract significantly reduced the mRNA and protein levels of ERα in all three ERα-positive cell lines. The downregulation of ERα protein levels was correlated with activation of the proteasomes. We demonstrated that Huaier extract markedly decreased the expression of both ERα and its downstream genes, inhibited the estrogen-stimulated proliferation and reversed the estrogen-induced activation of the nuclear factor κB (NFκB) pathway. Our study provides evidence that Huaier extract is a novel estrogen receptor modulator and is a promising drug for the prevention and treatment of ERα-positive human breast cancers. AD - Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China AU - Wang,Xiaolong AU - Zhang,Ning AU - Huo,Qiang AU - Sun,Mingjuan AU - Lv,Shangge AU - Yang,Qifeng DA - 2013/07/01 DO - 10.3892/ijo.2013.1947 EP - 328 IS - 1 JO - Int J Oncol KW - Huaier estrogen receptor nuclear factor κB breast cancer PY - 2013 SN - 1019-6439 1791-2423 SP - 321 ST - Huaier aqueous extract suppresses human breast cancer cell proliferation through inhibition of estrogen receptor α signaling T2 - International Journal of Oncology TI - Huaier aqueous extract suppresses human breast cancer cell proliferation through inhibition of estrogen receptor α signaling UR - https://doi.org/10.3892/ijo.2013.1947 VL - 43 ER -