TY - JOUR AB - We have demonstrated that an extract from the ripe fruit of Vitex agnus-castus (Vitex) exhibits cytotoxic activities against various types of solid tumor cells, whereas its effects on leukemia cells has not been evaluated to date. In this study, the effects of Vitex and its major component, casticin, on leukemia cell lines, HL-60 and U-937, were investigated by focusing on proliferation, induction of apoptosis and differentiation. Identification and quantitation by NMR spectroscopy showed that casticin accounted for approximate 1% weight of Vitex. Dose-dependent cytotoxicity of Vitex and casticin was observed in both cell lines, and HL-60 cells were more sensitive to the cytotoxicity of Vitex/casticin compared to U-937 cells. Furthermore, compared to unstimulated HL-60 cells, phorbol 12-myristate 13-acetate (PMA)- and 1,25-dihydroxyvitamin D3 (VD3)-differentiated HL-60 cells acquired resistance to Vitex/casticin based on the results from cell viability and apoptosis induction analysis. Since the HL-60 cell line is more immature than the U-937 cell line, these results suggested that the levels of cytotoxicity of Vitex/casticin were largely attributed to the degree of differentiation of leukemia cells; that is, cell lines with less differentiated phenotype were more susceptible than the differentiated ones. RT-PCR analysis demonstrated that PMA upregulated the expression of intercellular adhesion molecule-1 (ICAM-1) in HL-60 cells, and that anti-ICAM-1 monoclonal antibody not only abrogated PMA-induced aggregation and adhesion of the cells but also restored its sensitivity to Vitex. These results suggested that ICAM-1 plays a crucial role in the acquired resistance in PMA-differentiated HL-60 cells by contributing to cell adhesion. These findings provide fundamental insights into the clinical application of Vitex/casticin for hematopoietic malignancy. AD - Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan Yasuda Women's University, Faculty of Pharmacy, Asaminami-ku, Hiroshima 731-0153, Japan Department of Cultural History, Seisen University, Shinagawa-ku, Tokyo 141-0022, Japan Department of Clinical Pharmacology, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan Department of Applied Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan AU - Kikuchi,Hidetomo AU - Yuan,Bo AU - Nishimura,Yoshio AU - Imai,Masahiko AU - Furutani,Ryota AU - Kamoi,Saki AU - Seno,Misako AU - Fukushima,Shin AU - Hazama,Shingo AU - Hirobe,Chieko AU - Ohyama,Kunio AU - Hu,Xiao-Mei AU - Takagi,Norio AU - Hirano,Toshihiko AU - Toyoda,Hiroo DA - 2013/12/01 DO - 10.3892/ijo.2013.2133 EP - 1984 IS - 6 JO - Int J Oncol KW - Vitex agnus-castus casticin leukemic cells differ­entiation apoptosis induction intercellular adhesion molecule-1 PY - 2013 SN - 1019-6439 1791-2423 SP - 1976 ST - Cytotoxicity of Vitex agnus-castus fruit extract and its major component, casticin, correlates with differentiation status in leukemia cell lines T2 - International Journal of Oncology TI - Cytotoxicity of Vitex agnus-castus fruit extract and its major component, casticin, correlates with differentiation status in leukemia cell lines UR - https://doi.org/10.3892/ijo.2013.2133 VL - 43 ER -