TY - JOUR AB - Epidermal growth factor receptor (EGFR) signaling regulates glioblastoma cell proliferation, survival, migration and invasion and plays a key role in tumor progression. We show that microRNA-7 (miR-7) is a common regulator of the phosphoinositide-3-kinase (PI3K)/ATK and Raf/mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) pathways, both of which are launched by EGFR through its two direct targets, the transcription factors PI3K and Raf-1, respectively. Enforced expression of miR-7 markedly decreased expression of PI3K, phosphorylated Akt, Raf-1, phosphorylated MEK 1/2, and cyclin D1, as well as slightly reduced expression of EGFR. Forced expression of PI3K or Raf-1 transcripts lacking the 3'-untranslated region (3'-UTR) partially reversed the effects of miR-7 on cell growth inhibition and cell cycle arrest in glioma cells. Additionally, transient expression of miR-7 in glioblastoma cells strongly inhibited in vivo glioblastoma xenograft growth. We conclude that miR-7 is a potential tumor suppressor in glioblastoma that acts by targeting multiple oncogenes related to the downstream pathway of EGFR and may serve as a novel therapeutic target for malignant gliomas. AD - Department of Neurosurgery, The Fifth Central Hospital of Tianjin, Tianjin, P.R. China Department of Pathology, The Fifth Central Hospital of Tianjin, Tianjin, P.R. China Department of Biomedical Engineering, Duke University, Durham, NC, USA The Central Laboratory, The Fifth Central Hospital of Tianjin, Tianjin, P.R. China Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin, P.R. China AU - Liu,Zhenlin AU - Jiang,Zhongmin AU - Huang,Jianyong AU - Huang,Shuqiang AU - Li,Yanxia AU - Yu,Simiao AU - Yu,Shizhu AU - Liu,Xiaozhi DA - 2014/05/01 DO - 10.3892/ijo.2014.2322 EP - 1580 IS - 5 JO - Int J Oncol KW - microRNA-7 glioma epidermal growth factor receptor phosphoinositide-3-kinase PY - 2014 SN - 1019-6439 1791-2423 SP - 1571 ST - miR-7 inhibits glioblastoma growth by simultaneously interfering with the PI3K/ATK and Raf/MEK/ERK pathways T2 - International Journal of Oncology TI - miR-7 inhibits glioblastoma growth by simultaneously interfering with the PI3K/ATK and Raf/MEK/ERK pathways UR - https://doi.org/10.3892/ijo.2014.2322 VL - 44 ER -