TY - JOUR AB - We report a novel function of RUVBL1 molecule in pancreatic cancer cells. Previous reports describe that RUVBL1 belongs to the family of AAA+ ATPases that associate with chromatin-remodelling complexes and have important roles in transcriptional regulation, the DNA damage response, telomerase activity and cellular transformation. We show that knockdown of RUVBL1 inhibited the motility and invasiveness of pancreatic cancer cells. RUVBL1 localized in the cytoplasm bound filamentous actin (F-actin) in cell protrusions, and increased concentration of monomeric globular-actin (G-actin) in cell protrusions of migrating pancreatic cancer cells. Cytoplasmic RUVBL1 functioned in additional formation of actin filaments in cell protrusions. Consequently, cytoplasmic RUVBL1 contributed to the formation of membrane protrusions by promoting peripheral actin polymerization. Our results imply that these RUVBL1-actin interactions could enhance the invasive properties of pancreatic cancer cells. AD - Department of Pharmacology, School of Medicine, Kochi University, Nankoku, Kochi 783-8505, Japan Department of Pathology, School of Medicine, Kochi University, Nankoku, Kochi 783-8505, Japan Department of Gastroenterology and Hepatology, School of Medicine, Kochi University, Nankoku, Kochi 783-8505, Japan AU - Taniuchi,Keisuke AU - Furihata,Mutsuo AU - Iwasaki,Shinji AU - Tanaka,Kenjiro AU - Shimizu,Takahiro AU - Saito,Motoaki AU - Saibara,Toshiji DA - 2014/06/01 DO - 10.3892/ijo.2014.2380 EP - 1954 IS - 6 JO - Int J Oncol KW - pancreatic ductal adenocarcinoma AAA+ ATPase invasiveness cell protrusion actin polymerization PY - 2014 SN - 1019-6439 1791-2423 SP - 1945 ST - RUVBL1 directly binds actin filaments and induces formation of cell protrusions to promote pancreatic cancer cell invasion T2 - International Journal of Oncology TI - RUVBL1 directly binds actin filaments and induces formation of cell protrusions to promote pancreatic cancer cell invasion UR - https://doi.org/10.3892/ijo.2014.2380 VL - 44 ER -