TY - JOUR AB - Metastasis is the most insidious aspect of breast cancer, but effective strategies to control this malignant process are still lacking. In previous studies, we screened over 200 extracts from plants of genus Chloranthaceae by bioactivity-guided fractionation, and found that Codonolactone (CLT) exhibited potential antimetastatic properties in breast cancer cells. This sesquiterpene lactone was isolated from Chloranthus henryi Hemsl, and is also found in other medical herbs, such as Codonopsis pilosula, Atractylodes macrocephala Koidz and others. Here, we report that CLT inhibited the ability of invasion and migration in metastatic breast cancer cells. Furthermore, CLT exhibited significant suppression on formation of lung metastatic foci of breast cancer in vivo. We next investigated the mechanism of CLT-induced metastasis inhibitory effects in breast cancer cells. A significant inhibition on activity and expression of MMP-9 and MMP-13 was observed. Moreover, data from western blotting, Runx2 transcription factor assay and chromatin immunoprecipitation assay showed that binding ability of Runx2 to sequences of the mmp-13 promoter was inhibited by CLT. Collectively, these findings suggested that the antimetastatic properties of CLT in breast cancer were due to the inhibition of MMPs, which might be associated with a downregulation of Runx2 transcriptional activity. AD - Department of Pharmacology, School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, P.R. China Hospital of Nanchang University, Nanchang 330047, P.R. China Network and Educational Technology Center, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, P.R. China AU - Wang,Wei AU - Chen,Bin AU - Zou,Ruolan AU - Tu,Xiuying AU - Tan,Songlin AU - Lu,Hong AU - Liu,Zhaojie AU - Fu,Jianjiang DA - 2014/11/01 DO - 10.3892/ijo.2014.2643 EP - 1900 IS - 5 JO - Int J Oncol KW - Codonolactone invasion migration metastasis Runx2 breast cancer PY - 2014 SN - 1019-6439 1791-2423 SP - 1891 ST - Codonolactone, a sesquiterpene lactone isolated from Chloranthus henryi Hemsl, inhibits breast cancer cell invasion, migration and metastasis by downregulating the transcriptional activity of Runx2 T2 - International Journal of Oncology TI - Codonolactone, a sesquiterpene lactone isolated from Chloranthus henryi Hemsl, inhibits breast cancer cell invasion, migration and metastasis by downregulating the transcriptional activity of Runx2 UR - https://doi.org/10.3892/ijo.2014.2643 VL - 45 ER -