TY - JOUR AB - Lung cancer is the leading cause of cancer‑related deaths worldwide. Epidermal growth factor receptor‑tyrosine kinase inhibitors (EGFR‑TKIs), such as gefitinib and erlotinib, have demonstrated marked clinical activity against non‑small cell lung cancer (NSCLC) harboring activating epidermal growth factor receptor (EGFR) mutations. However, in most cases, patients develop acquired resistance to EGFR‑TKI therapy. The threonine to methionine change at codon 790 of EGFR (EGFR T790M) mutation is the most common acquired resistance mutation, and is present in ~50% cases of TKI resistance. New treatment strategies for NSCLC patients harboring the EGFR T790M mutation are required. We evaluated the immunogenicity of an antigen derived from EGFR with the T790M mutation. Using BIMAS we selected several EGFR T790M‑derived peptides bound to human leukocyte antigen (HLA)‑A*02:01. T790M‑A peptide (789‑797) (IMQLMPFGC)‑specific cytotoxic T lymphocytes (CTLs) were induced from peripheral blood mononuclear cells (PBMCs) of HLA‑A2+ healthy donors. An established T790M‑A‑specific CTL line showed reactivity against the NCSLC cell line, H1975‑A2 (HLA‑A2+, T790M+), but not H1975 (HLA‑A2‑, T790M+), and the corresponding wild‑type peptide (ITQLMPFGC)‑pulsed T2 cells using an interferon‑γ (IFN‑γ) enzyme‑linked immuno spot (ELISPOT) assay. This CTL line also demonstrated peptide‑specific cytotoxicity against H1975‑A2 cells. This finding suggests that the EGFR T790M mutation‑derived antigen could be a new target for cancer immunotherapy. AD - Division of Cancer Immunotherapy, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Chiba, Japan Second Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Fukui, Japan AU - Ofuji,Kazuya AU - Tada,Yoshitaka AU - Yoshikawa,Toshiaki AU - Shimomura,Manami AU - Yoshimura,Mayuko AU - Saito,Keigo AU - Nakamoto,Yasunari AU - Nakatsura,Tetsuya DA - 2015/02/01 DO - 10.3892/ijo.2014.2787 EP - 504 IS - 2 JO - Int J Oncol KW - acquired resistance CTL epitope EGFR T790M immunotherapy non‑small cell lung cancer PY - 2015 SN - 1019-6439 1791-2423 SP - 497 ST - A peptide antigen derived from EGFR T790M is immunogenic in non‑small cell lung cancer T2 - International Journal of Oncology TI - A peptide antigen derived from EGFR T790M is immunogenic in non‑small cell lung cancer UR - https://doi.org/10.3892/ijo.2014.2787 VL - 46 ER -