TY - JOUR AB - MicroRNAs are a family of small non-coding RNAs that constitute a prevalent gene regulation. In this study, we showed the expression of miR-512-5p is downregulated in non-small cell lung cancer (NSCLC) patient tumor samples compared to its paired normal lung tissues. Moreover, expression of miR-512-5p was increased by retinoic acid treatment. Overexpression of miR-512-5p induced apoptosis of NSCLC cell lines A549 and H1299, and miR-512-5p inhibitor reversed this effect in H1299 cells stably expressing miR-512. miR-512-5p inhibited glycolysis and migration in NSCLC cells, but shows no effect on cell proliferation. We identified p21 as a target gene of miR-512-5p. Overexpression of miR-512-5p led to the decrease of p21 protein and mRNA level. Knockdown of p21 resulted in similar effects on apoptosis and glycolysis as that observed of miR-512-5p overexpression, as well as rescued the effect of miR-512-5p inhibitor on cell apoptosis in H1299 cells stably expressing miR-512. In conclusion, our present study revealed miR-512-5p was able to target p21 to induce apoptosis and inhibit glycolysis in A549 and H1299 cell lines. AD - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, P.R. China Department of Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Tongji University Cancer Institute, Shanghai 200433, P.R. China AU - Chu,Kaili AU - Gao,Guanghui AU - Yang,Xiufang AU - Ren,Shengxiang AU - Li,Yao AU - Wu,Hai AU - Huang,Yan AU - Zhou,Caicun DA - 2016/02/01 DO - 10.3892/ijo.2015.3279 EP - 586 IS - 2 JO - Int J Oncol KW - miR-512-5p p21 apoptosis glycolysis migration PY - 2016 SN - 1019-6439 1791-2423 SP - 577 ST - miR-512-5p induces apoptosis and inhibits glycolysis by targeting p21 in non-small cell lung cancer cells T2 - International Journal of Oncology TI - miR-512-5p induces apoptosis and inhibits glycolysis by targeting p21 in non-small cell lung cancer cells UR - https://doi.org/10.3892/ijo.2015.3279 VL - 48 ER -