TY - JOUR AB - Mantle cell lymphoma (MCL) is an aggressive B cell lymphoma with a poor prognosis. It is characterized by the t(11;14)(q13;q32) translocation, resulting in over­expression of CCND1. Morphologically, MCL is categorised into two types: classical MCL (cMCL) and aggressive MCL (aMCL), with a proportion of cMCL progressing to develop into aMCL. miRNAs are currently considered to be important regulators for cell behavior and are deregulated in many malignancies. Although several genetic alterations have been implicated in the transformation of cMCL to aMCL, the involvement of miRNAs in transformation is not known. In an effort to identify the miRNAs related to the transformation of MCL, miRNA microarray analyses were used for cMCL and aMCL cases. These analyses demonstrated significant differences in the expression of seven microRNAs based on a t-test (p-value <0.05); miR-15b was greatly upregulated in aMCL. Locked nucleic acid in situ hybridization showed increased staining of miR-15b in formalin-fixed paraffin-embedded sections of aMCL. These results correlated well with the microRNA microarray analysis. Although the molecular functions of miR-15b are largely unknown, it has been found to be associated with the cell cycle and apoptosis. However, the physiological significance of increased miR-15b in MCL is still unknown. Our present findings suggest that the upregulated expression of miR-15b is likely to play an important role in the trans­formation of cMCL to aMCL. AD - Department of Pathology, School of Medicine, Kurume University, Kurume, Japan Cell Innovator Inc., Venture Business Laboratory of Kyushu University, Fukuoka, Japan Laboratory of Molecular Gene Technology and Molecular Biosciences, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan AU - Arakawa,Fumiko AU - Kimura,Yoshizo AU - Yoshida,Noriaki AU - Miyoshi,Hiroaki AU - Doi,Atushi AU - Yasuda,Kaori AU - Nakajima,Kazutaka AU - Kiyasu,Junichi AU - Niino,Daisuke AU - Sugita,Yasuo AU - Tashiro,Kosuke AU - Kuhara,Satoru AU - Seto,Masao AU - Ohshima,Koichi DA - 2016/02/01 DO - 10.3892/ijo.2015.3295 EP - 492 IS - 2 JO - Int J Oncol KW - mantle cell lymphoma laser microdissection miRNA microarray in situ hybridization miR-15b PY - 2016 SN - 1019-6439 1791-2423 SP - 485 ST - Identification of miR-15b as a transformation-related factor in mantle cell lymphoma T2 - International Journal of Oncology TI - Identification of miR-15b as a transformation-related factor in mantle cell lymphoma UR - https://doi.org/10.3892/ijo.2015.3295 VL - 48 ER -