TY - JOUR AB - Breast cancer is the most common cancer in women. Although advances in diagnostic imaging for early detection, surgical techniques and chemotherapy have improved overall survival, the prognosis of patients with metastatic breast cancer remains poor. Understanding cancer cell dynamics in the metastatic process is important to develop new therapeutic strategies. Experimental animal models and imaging would be powerful tools for understanding of the molecular events of multistep process of metastasis. In the present study, to develop a new cancer cell line that is applicable to bioluminescence and fluorescence imaging, we transfected the expression vector of a green fluorescent protein ZsGreen1 into a metastatic cell line 5a-D-Luc, which is a subclone of the MDA-MB-231 breast cancer cell line expressing luciferase, and established a new tumor cell line 5a-D-Luc-ZsGreen expressing both luciferase and ZsGreen1. The 5a-D-Luc-ZsGreen cells proliferate more rapidly and have a more invasive phenotype compared with 5a-D-Luc cells following intracardiac injection. Metastasis sites were easily detected in the whole body by bioluminescence imaging and in excised tissues by ex vivo fluorescence imaging. The fluorescence of 5a-D-Luc-ZsGreen cells was not lost after formalin fixation and decalcification. It enabled us to easily evaluate tumor spread and localization at the cellular level in microscopic analysis. The strong fluorescence of 5a-D-Luc-ZsGreen cells allowed for real-time imaging of circulating tumor cells in cerebral blood vessels of live animals immediately after intracardiac injection of cells using two-photon laser-scanning microscopy. These findings suggest that the 5a-D-Luc-ZsGreen cells would be a useful tool for research on mechanisms of metastatic process in animal models. AD - Diagnostic Imaging Program, Molecular Imaging Center, National Institute of Radiological Sciences, Inage-ku, Chiba 263-8555, Japan Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, Inage-ku, Chiba 263-8555, Japan Center for Frontier Science and Engineering, University of Electro-Communications, Chofu, Tokyo 182-8585, Japan Department of Neurology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan Department of Molecular Medicine for Pathogenesis, Ehime University Graduate School of Medicine, Ehime 791-0295, Japan Department of Nuclear Medicine, Cancer Institute Hospital of Japanese Foundation for Cancer Research, Koto-ku, Tokyo 135-8550, Japan AU - Sudo,Hitomi AU - Tsuji,Atsushi ,B. AU - Sugyo,Aya AU - Takuwa,Hiroyuki AU - Masamoto,Kazuto AU - Tomita,Yutaka AU - Suzuki,Norihiro AU - Imamura,Takeshi AU - Koizumi,Mitsuru AU - Saga,Tsuneo DA - 2016/02/01 DO - 10.3892/ijo.2015.3300 EP - 532 IS - 2 JO - Int J Oncol KW - bone metastasis brain metastasis animal model biolumi­nescence imaging fluorescence imaging in vivo imaging real-time imaging two-photon excitation microscopy PY - 2016 SN - 1019-6439 1791-2423 SP - 525 ST - Establishment and evaluation of a new highly metastatic tumor cell line 5a-D-Luc-ZsGreen expressing both luciferase and green fluorescent protein T2 - International Journal of Oncology TI - Establishment and evaluation of a new highly metastatic tumor cell line 5a-D-Luc-ZsGreen expressing both luciferase and green fluorescent protein UR - https://doi.org/10.3892/ijo.2015.3300 VL - 48 ER -