TY - JOUR AB - Metastasis is the main cause of cancer-associated death, and metastasis of pancreatic cancer remains difficult to treat because of its aggressiveness. MicroRNAs (miRNAs) play crucial roles in the regulation of various human transcripts, and many miRNAs have been reported to correlate with cancer metastasis. We identified an anti-metastatic miRNA, miR-5100, by investigating differences in miRNA profiling between highly metastatic pancreatic cancer cells and their parental cells. Overexpression of miR-5100 inhibited colony formation (P<0.05), cell migration (P<0.0001) and invasion (P<0.0001) of pancreatic cancer cells. In addition, we identified a possible target of miR-5100, podocalyxin-like 1 (PODXL), and demonstrated miR-5100 directly binds to the 3' untranslated region of PODXL and post-transcriptionally regulates its expression in pancreatic cancer cells. Silencing PODXL resulted in diminished cell migration (P<0.0001) and invasion (P<0.05). We also clarified the close relationship between expression of PODXL in human pancreatic cancer specimens and liver metastasis (P=0.0003), and determined that post-operative survival was longer in the low-PODXL expression group than in the high-PODXL expression group (P<0.05). These results indicate that miR-5100 and PODXL have considerable therapeutic potential for anti-metastatic therapy and could be potential indicators for cancer metastases in patients with pancreatic cancer. AD - Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan Kyushu University Hospital Cancer Center, Fukuoka, Japan AU - Chijiiwa,Yoshiro AU - Moriyama,Taiki AU - Ohuchida,Kenoki AU - Nabae,Toshinaga AU - Ohtsuka,Takao AU - Miyasaka,Yoshihiro AU - Fujita,Hayato AU - Maeyama,Ryo AU - Manabe,Tatsuya AU - Abe,Atsushi AU - Mizuuchi,Yusuke AU - Oda,Yoshinao AU - Mizumoto,Kazuhiro AU - Nakamura,Masafumi DA - 2016/04/01 DO - 10.3892/ijo.2016.3389 EP - 1700 IS - 4 JO - Int J Oncol KW - pancreatic cancer miR-5100 PODXL liver metastasis distant metastasis prognostic marker PY - 2016 SN - 1019-6439 1791-2423 SP - 1688 ST - Overexpression of microRNA-5100 decreases the aggressive phenotype of pancreatic cancer cells by targeting PODXL T2 - International Journal of Oncology TI - Overexpression of microRNA-5100 decreases the aggressive phenotype of pancreatic cancer cells by targeting PODXL UR - https://doi.org/10.3892/ijo.2016.3389 VL - 48 ER -