TY - JOUR AB - Citrus platymamma Hort.et Tanaka is an indigenous fruit of Jeju island in Korea. In this study the bioactivity of C. platymamma flavonoids were evaluated on human hepatoma Hep3B cell lines. Eleven flavonoids were identified from the peels of C. platymamma Hort.et Tanaka through high-performance liquid chromatography-Tandem mass spectrometry and the anticancer effect of these C. platymamma flavonoids on human hepatoma Hep3B were studied. Chromatin condensation was observed in Hep3B cells treated with C. platymamma flavonoids. DNA fragmentation was confirmed through agarose gel electrophoresis and TUNEL assay. An increase in the total apoptotic cells and G2/M cell cycle arrest with decreased protein expression of CDC25C, CDK1, cyclin B1 and p21 were observed in Hep3B cells treated with flavonoids of C. platymamma. Further, protein expression of Bcl-XL, Bax, caspase-3 and -9 were also modulated by C. platymamma flavonoids treatment indicating that cell death is through intrinsic apoptotic pathway. Moreover, C. platymamma flavonoids also regulated the phosphorylation of MAPKs, PI3K, and Akt in Hep3B cells. Relevant to inhibiting metastasis, C. platymamma treatment reduced wound closure of Hep3B cells and the protein expression of matrix metalloproteinase-2 and -9 were reduced in C. platymamma treated cells. The results show that C. platymamma flavonoids induce cell cycle arrest and apoptosis following activation of MAPKs and suppression of PI3K/Akt pathway which eventually inhibits cell migration in Hep3B cells. The finding provides evidence on biochemical activities of C. platymamma Hort.et Tanaka, which would be an essential agent for hepatocellular carcinoma (HCC) treatment. AD - Research Institute of Life Science and College of Veterinary Medicine, Gyeongsang National University, Gazwa, Jinju 660-701, Republic of Korea Department of Physical Therapy, International University of Korea, Jinju 660-759, Republic of Korea Gyeongnam Biological Resource Research Center, Korea Institute of Toxicology, Jinju 666-844, Republic of Korea Department of Nursing Science, International University of Korea, Jinju 660-759, Republic of Korea College of Veterinary Medicine, Gyeongsang National University, Gazwa, Jinju 660-701, Republic of Korea AU - Hong,Gyeong,Eun AU - Lee,Ho,Jeong AU - Kim,Jin,A. AU - Yumnam,Silvia AU - Raha,Suchismita AU - Venkatarame Gowda Saralamma,Venu AU - Heo,Jeong,Doo AU - Lee,Sang,Joon AU - Kim,Eun,Hee AU - Won,Chun,Kil AU - Kim,Gon,Sup DA - 2017/02/01 DO - 10.3892/ijo.2016.3816 EP - 586 IS - 2 JO - Int J Oncol KW - Hep3B apoptosis metastasis C. platymamma flavonoids caspase PY - 2017 SN - 1019-6439 1791-2423 SP - 575 ST - Korean Byungkyul - Citrus platymamma Hort.et Tanaka flavonoids induces cell cycle arrest and apoptosis, regulating MMP protein expression in Hep3B hepatocellular carcinoma cells T2 - International Journal of Oncology TI - Korean Byungkyul - Citrus platymamma Hort.et Tanaka flavonoids induces cell cycle arrest and apoptosis, regulating MMP protein expression in Hep3B hepatocellular carcinoma cells UR - https://doi.org/10.3892/ijo.2016.3816 VL - 50 ER -