TY - JOUR AB - MicroRNAs (miRNAs) are a large family of small, non-coding RNAs that play a pivotal role in tumorigenesis. miR‑34a, which is a member of the miR-34 family, is a downstream target of p53. Increasing evidence shows that miR-34a dysregulation may contribute to tumor development and progression in numerous cancers, including esophageal squamous cell carcinoma (ESCC). However, the mechanism of miR-34a in the regulation of ESCC cells need to be further elucidated because of the complex regulative network of miRNAs. The miR-34a expression in ESCC samples has been confirmed using quantitative reverse transcription polymerase chain reaction. The effects of miR-34a on cell migration and invasion were examined in ESCC cell lines using wound healing and Transwell assays, respectively. The effects of miR-34a on matrix metalloproteinase (MMP)-2 and -9 and fibronectin type III domain containing 3B (FNDC3B) expression levels were detected by luciferase reporter assays and western blot analysis. Quantitative polymerase chain reaction revealed that the miR‑34a expression is significantly downregulated in the ESCC tissues compared to that in the adjacent normal tissues. miR-34a overexpression was significantly suppressed migration and invasion in the ESCC cells and simultaneously inhibited the MMP-2, MMP-9 and FNDC3B expression levels by targeting the coding and 3'-untranslated regions, respectively. The findings indicated that microRNA‑34a suppresses cell migration and invasion by targeting MMP-2, MMP-9, and FNDC3B in ESCC. AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, P.R. China The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, P.R. China AU - Yang,Lan AU - Song,Xiaoyue AU - Zhu,Jianbo AU - Li,Mei AU - Ji,Yu AU - Wu,Fei AU - Chen,Yunzhao AU - Cui,Xiaobin AU - Hu,Jianming AU - Wang,Lianghai AU - Cao,Yuwen AU - Wei,Yutao AU - Zhang,Wenjie AU - Li,Feng DA - 2017/07/01 DO - 10.3892/ijo.2017.4015 EP - 388 IS - 1 JO - Int J Oncol KW - esophageal squamous cell carcinoma miR-34a migration and invasion matrix metalloproteinase 2/9 fibronectin type III domain containing 3B PY - 2017 SN - 1019-6439 1791-2423 SP - 378 ST - Tumor suppressor microRNA-34a inhibits cell migration and invasion by targeting MMP-2/MMP-9/FNDC3B in esophageal squamous cell carcinoma T2 - International Journal of Oncology TI - Tumor suppressor microRNA-34a inhibits cell migration and invasion by targeting MMP-2/MMP-9/FNDC3B in esophageal squamous cell carcinoma UR - https://doi.org/10.3892/ijo.2017.4015 VL - 51 ER -