TY - JOUR AB - MicroRNAs (miRNAs or miRs) play important roles in carcinogenesis. The miRNA, miR-205-5p, has been reported to suppress the growth of various types of tumor; however, its functional contribution to oral squamous cell carcinoma (OSCC) is not yet clear. Thus, this study was conducted to determine the miRNA expression signatures in OSCC and to investigate the functional role of miR‑205‑5p in OSCC cells. We measured miR‑205‑5p expression by RT-qPCR, and examined the function of miR‑205‑5p by transfecting a miR‑205‑5p mimic or inhibitor into OSCC cells and measuring cell proliferation, migration and invasiveness. Genes targeted by miR‑205‑5p were identified using the TargetScan database and verified by western blot analysis, luciferase reporter assay and ELISA. We found that miR‑205‑5p was significantly downregulated in OSCC cell lines and tissue specimens. Following transfection of miR‑205‑5p mimic or inhibitor into the cancer cell lines, miR‑205‑5p overexpression significantly suppressed cancer cell migration and invasion. We further demonstrated that miR‑205‑5p directly targeted and regulated the tissue inhibitor of metalloproteinases‑2 (TIMP‑2) gene. The silencing of TIMP‑2 suppressed cancer cell invasion and the activation of pro‑matrix metalloproteinase‑2 (pro‑MMP‑2). These results suggest that TIMP‑2 promotes tumor progression, and that miR‑205‑5p directly regulates TIMP‑2, thereby suppressing pro‑MMP‑2 activation and inhibiting OSCC cell invasiveness. Our data describing the pathways regulated by miR‑205‑5p provide new insight into the mechanisms responsible for OSCC development and metastasis. AD - Department of Oral and Maxillofacial Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8571, Japan Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8571, Japan Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Ishikawa 920-0934, Japan Cardiovascular Division, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8571, Japan Department of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Kanazawa, Ishikawa 920-1192, Japan AU - Nagai,Hiroki AU - Hasegawa,Shogo AU - Uchida,Fumihiko AU - Terabe,Takehito AU - Ishibashi Kanno,Naomi , AU - Kato,Koroku AU - Yamagata,Kenji AU - Sakai,Satoshi AU - Kawashiri,Shuichi AU - Sato,Hiroshi AU - Yanagawa,Toru AU - Bukawa,Hiroki DA - 2018/03/01 DO - 10.3892/ijo.2018.4260 EP - 850 IS - 3 JO - Int J Oncol KW - invasion microRNA‑205‑5p matrix metalloproteinase‑2 activation oral squamous cell carcinoma tissue inhibitor of metalloproteinases‑2 PY - 2018 SN - 1019-6439 1791-2423 SP - 841 ST - MicroRNA-205-5p suppresses the invasiveness of oral squamous cell carcinoma by inhibiting TIMP‑2 expression T2 - International Journal of Oncology TI - MicroRNA-205-5p suppresses the invasiveness of oral squamous cell carcinoma by inhibiting TIMP‑2 expression UR - https://doi.org/10.3892/ijo.2018.4260 VL - 52 ER -