TY - JOUR AB - Non-small cell lung cancer (NSCLC) is considered to be an inflammation-associated carcinoma. Although interleukin‑17 (IL‑17) production contributes to the proliferation and growth of NSCLC, the mechanisms underlying IL‑17-induced NSCLC cell proliferation have not been fully elucidated. In the present study, by using ELISA and immunohistochemical analyses, we first found that the expression levels of IL‑17, IL‑17 receptor (IL‑17R), high-mobility group A1 (HMGA1) and cyclin D1 were elevated in the samples of patients with NSCLC. Subsequently, by RT-qPCR, western blot analysis and cell proliferation assay in vitro, we revealed that stimulation with recombinant human IL‑17 (namely IL‑17A) markedly induced the expression of HMGA1 and cyclin D1 in the A549 cells (a human lung adenocarcinoma cell line) and promoted cell proliferation. Furthermore, luciferase reporter and ChIP assays confirmed that upregulated HMGA1 directly bound to the cyclin D1 gene promoter and activated its transcription. Notably, the response element of HMGA1 binding to the cyclin D1 promoter was disclosed for the first time, at least to the best of our knowledge. Taken together, our findings indicate that the IL‑17/HMGA1/cyclin D1 axis plays an important role in NSCLC cell proliferation and may provide new insight into NSCLC pathogenesis and may thus aid in the development of novel therapeutic targets for NSCLC. AD - Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210009, P.R. China Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China Department of Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210009, P.R. China AU - Zhao,Chenhui AU - Li,Yongting AU - Zhang,Weiming AU - Zhao,Dan AU - Ma,Ling AU - Ma,Pei AU - Yang,Fengming AU - Wang,Yingwei AU - Shu,Yongqian AU - Qiu,Wen DA - 2018/05/01 DO - 10.3892/ijo.2018.4307 EP - 1592 IS - 5 JO - Int J Oncol KW - non-small cell lung cancer interleukin 17 high-mobility group A1 cyclin D1 proliferation PY - 2018 SN - 1019-6439 1791-2423 SP - 1579 ST - IL‑17 induces NSCLC A549 cell proliferation via the upregulation of HMGA1, resulting in an increased cyclin D1 expression T2 - International Journal of Oncology TI - IL‑17 induces NSCLC A549 cell proliferation via the upregulation of HMGA1, resulting in an increased cyclin D1 expression UR - https://doi.org/10.3892/ijo.2018.4307 VL - 52 ER -