TY - JOUR AB - Resveratrol (RSV) is a polyphenolic compound that naturally occurs in grapes, peanuts and berries. Considerable research has been conducted to determine the benefits of RSV against various human cancer types. Tristetraprolin (TTP) is an AU-rich element-binding protein that regulates mRNA stability and has decreased expression in human cancer. The present study investigated the biological effect of RSV on TTP gene regulation in colon cancer cells. RSV inhibited the proliferation and invasion/metastasis of HCT116 and SNU81 colon cancer cells. Furthermore, RSV induced a dose-dependent increase in TTP expression in HCT116 and SNU81 cells. The microarray experiment revealed that RSV significantly increased TTP expression by downregulating E2F transcription factor 1 (E2F1), a downstream target gene of TTP and regulated genes associated with inflammation, cell proliferation, cell death, angiogenesis and metastasis. Although TTP silencing inhibited TTP mRNA expression, the expression was subsequently restored by RSV. Small interfering RNA-induced TTP inhibition attenuated the effects of RSV on cell growth. In addition, RSV induced the mRNA-decaying activity of TTP and inhibited the relative luciferase activity of baculoviral IAP repeat containing 3 (cIAP2), large tumor suppressor kinase 2 (LATS2), E2F1, and lin‑28 homolog A (Lin28) in HCT116 and SNU81 cells. Therefore, RSV enhanced the inhibitory activity of TTP in HCT116 and SNU81 cells by negatively regulating cIAP2, E2F1, LATS2, and Lin28 expression. In conclusion, RSV suppressed the proliferation and invasion/metastasis of colon cancer cells by activating TTP. AD - Department of Biological Science, Dong-A University, Busan 49315, Republic of Korea Department of Physiology, Institute of Medical Science, Chonbuk National University Medical School, Jeonju, Jeonbuk 54907, Republic of Korea AU - Lee,Se-Ra AU - Jin,Hua AU - Kim,Won-Tae AU - Kim,Won-Jung AU - Kim,Sung Zoo AU - Leem,Sun-Hee AU - Kim,Soo Mi DA - 2018/09/01 DO - 10.3892/ijo.2018.4453 EP - 1278 IS - 3 JO - Int J Oncol KW - resveratrol colon cancer cells tristetraprolin metastasis invasion cell proliferation PY - 2018 SN - 1019-6439 1791-2423 SP - 1269 ST - Tristetraprolin activation by resveratrol inhibits the proliferation and metastasis of colorectal cancer cells T2 - International Journal of Oncology TI - Tristetraprolin activation by resveratrol inhibits the proliferation and metastasis of colorectal cancer cells UR - https://doi.org/10.3892/ijo.2018.4453 VL - 53 ER -