TY - JOUR AB - Glucose-6-phosphate dehydrogenase (G6PD) is a rate-limiting enzyme of the pentose phosphate pathway. Multiple studies have previously revealed that elevated G6PD levels promote cancer progression in numerous tumor types; however, the underlying mechanism remains unclear. In the present study, it was demonstrated that high G6PD expression is a poor prognostic factor in bladder cancer, and the levels of G6PD expression increase with increasing tumor stage. Patients with bladder cancer with high G6PD expression had worse survival rates compared with those with lower G6PD expression in resected tumors. In vitro experiments revealed that knockdown of G6PD suppressed cell viability and growth in Cell Counting Kit-8 and colony formation assays, and increased apoptosis in bladder cancer cell lines compared with normal cells. Further experiments indicated that the weakening of the survival ability in G6PD-knockdown bladder cancer cells may be explained by intracellular reactive oxygen species accumulation and protein kinase B pathway suppression. Furthermore, it was additionally revealed that 6-aminonicotinamide (6-AN), a competitive G6PD inhibitor, may be a potential therapy for bladder cancer, particularly in cases with high G6PD expression, and that the combination of cisplatin and 6-AN may optimize the clinical dose or minimize the side effects of cisplatin. AD - Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, P.R. China AU - Chen,Xiaoyi AU - Xu,Zhijie AU - Zhu,Zhijian AU - Chen,Anqi AU - Fu,Guanghou AU - Wang,Yimin AU - Pan,Hao AU - Jin,Baiye DA - 2018/10/01 DO - 10.3892/ijo.2018.4501 EP - 1712 IS - 4 JO - Int J Oncol KW - glucose-6-phosphate dehydrogenase bladder cancer reactive oxygen species accumulation protein kinase B pathway 6-aminonicotinamide cisplatin PY - 2018 SN - 1019-6439 1791-2423 SP - 1703 ST - Modulation of G6PD affects bladder cancer via ROS accumulation and the AKT pathway in vitro T2 - International Journal of Oncology TI - Modulation of G6PD affects bladder cancer via ROS accumulation and the AKT pathway in vitro UR - https://doi.org/10.3892/ijo.2018.4501 VL - 53 ER -