TY - JOUR AB - Dysregulated cell cycle progression serves a crucial role in tumor development. Cell division cycle-associated 3 (CDCA3) is considered a trigger of mitotic entry; it is an important part of the S phase kinase-associated protein 1/Cullin/F-box ubiquitin ligase complex and mediates the destruction of mitosis-inhibitory kinase wee1. However, little is known about the role of CDCA3 in cancer, particularly colorectal cancer (CRC). The present study aimed to explore the biological and clinical significance of CDCA3 in CRC growth and progression. CDCA3 expression was significantly associated with tumor progression and poor survival. Overexpression of CDCA3 increased proliferation in LoVo CRC cells, whereas CDCA3 knockdown in SW480 CRC cells led to decreased proliferation, in vitro and in vivo. Further mechanistic investigations demonstrated that reduced CDCA3 expression resulted in G1/S phase transition arrest, which was attributed to a significant accumulation of p21 in SW480 cells; conversely, increased CDCA3 expression promoted G1/S phase transition through decreased p21 accumulation in LoVo cells. It was also demonstrated that CDCA3 was able to regulate the expression of transcription factor E2F1, thereby repressing p21 expression. Taken together, these results suggested that overexpression of CDCA3 may serve a crucial role in tumor malignant potential and that CDCA3 may be used as a prognostic factor and a potential therapeutic target in CRC. AD - The First School of Clinical Medicine, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China AU - Qian,Wenwei AU - Zhang,Zhiyuan AU - Peng,Wen AU - Li,Jie AU - Gu,Qiou AU - Ji,Dongjian AU - Wang,Qingyuan AU - Zhang,Yue AU - Ji,Bing AU - Wang,Sen AU - Zhang,Dongsheng AU - Sun,Yueming DA - 2018/11/01 DO - 10.3892/ijo.2018.4538 EP - 2033 IS - 5 JO - Int J Oncol KW - colorectal cancer cell division cycle-associated 3 transcription factor E2F1 p21 cell cycle cell proliferation oncogene PY - 2018 SN - 1019-6439 1791-2423 SP - 2021 ST - CDCA3 mediates p21-dependent proliferation by regulating E2F1 expression in colorectal cancer T2 - International Journal of Oncology TI - CDCA3 mediates p21-dependent proliferation by regulating E2F1 expression in colorectal cancer UR - https://doi.org/10.3892/ijo.2018.4538 VL - 53 ER -