TY - JOUR AB - In the present study, in order to identify novel diagnostic biomarkers for the malignant behavior of oral squamous cell carcinoma (OSCC), we determined the proteomic profiles of several OSCC cell lines and keratinocytes by two-dimensional fluorescence difference gel electrophoresis and liquid chromatography tandem mass spectrometry. The protein expression level of α-1-microglobulin/bikunin precursor (AMBP) was found to be significantly lower in the OSCC cell lines than in the keratinocytes, and a significant decrease in AMBP mRNA expression was confirmed in the OSCC cell lines by RT-qPCR. To investigate the biological function of AMBP in OSCC, the cells were transiently transfected with an AMBP overexpression vector; the AMBP-overexpressing cells exhibited a significantly decreased invasion and migration in comparison to the mock-transfected control cells, although no significant changes in cell proliferation were observed. Immunohistochemistry revealed that the underexpression of AMBP was significantly associated with a high metastatic potential to cervical lymph nodes and a poor overall survival. Thus, the expression of AMBP is an independent predictive factor of cervical lymph node metastasis and a prognostic factor of overall survival, and it is involved in both cell invasion and metastasis in cervical lymph nodes in OSCC. AD - Department of Oral and Maxillofacial Surgery, Tokyo Dental College, Chiba 261-8502, Japan Department of Biomarkers for Early Detection of Cancer, National Cancer Center Research Institute, Tokyo 104-0045, Japan Department of Oral Medicine, Oral and Maxillofacial Surgery, Tokyo Dental College, Chiba 272-8513, Japan Oral Cancer Center, Tokyo Dental College, Chiba 272-8513, Japan AU - Sekikawa,Shoichi AU - Onda,Takeshi AU - Miura,Nami AU - Nomura,Takeshi AU - Takano,Nobuo AU - Shibahara,Takahiko AU - Honda,Kazufumi DA - 2018/12/01 DO - 10.3892/ijo.2018.4581 EP - 2614 IS - 6 JO - Int J Oncol KW - oral cancer squamous cell carcinoma prognostic biomarker α-1-microglobulin/bikunin precursor two-dimensional fluorescence difference gel electrophoresis PY - 2018 SN - 1019-6439 1791-2423 SP - 2605 ST - Underexpression of α-1-microglobulin/bikunin precursor predicts a poor prognosis in oral squamous cell carcinoma T2 - International Journal of Oncology TI - Underexpression of α-1-microglobulin/bikunin precursor predicts a poor prognosis in oral squamous cell carcinoma UR - https://doi.org/10.3892/ijo.2018.4581 VL - 53 ER -