TY - JOUR AB - Wilms' tumor is one of the most common malignant tumors of the abdomen in children. However, there is currently no recognized specific biomarker for the clinical diagnosis and prognosis of this tumor. Lipid metabolism is involved in membrane synthesis and oxidation in tumor cells. This process plays an important role in the development of tumors, but it has not yet been investigated in Wilms' tumor. The aim of the present study was to characterize the changes in lipid metabolism and to contribute to the diagnosis and prognosis of Wilms' tumor. Proteomics analysis was performed to detect lipid‑metabolizing enzymes in 9 tissue samples from Wilms' tumors and adjacent tissues, and proteomics revealed the presence of 19 differentially expressed lipid‑metabolizing enzymes. Protein interaction analysis with the Search Tool for the Retrieval of Interacting Genes/Proteins was used to identify the interacting proteins. Immunohistochemistry (IHC), immunofluorescence and western blotting were used to further confirm whether the expression of fatty acid synthase (FASN) was significantly increased in the tumor tissues. Oncomine database and reverse transcription‑PCR analyses further confirmed that the expression of FASN at the gene level was significantly increased in the tumors. Following collection of 65 pediatric cases of Wilms' tumor at the Shandong Provincial Hospital between 2007 and 2012, the association between the expression of FASN and the clinical characteristics was analyzed, and IHC analysis further demonstrated that FASN expression was significantly associated with tumor stage and size. The association between FASN and the prognosis of children with Wilms' tumor was analyzed using Kaplan‑Meier survival curves. In addition, univariate survival analysis revealed that higher expression of FASN in Wilms' tumors was associated with poorer prognosis. Our findings revealed that FASN may be used as a prognostic biomarker in patients with Wilms' tumor. Furthermore, lipid metabolism may play an important role in the occurrence and development of Wilms' tumor. AD - Department of Pediatric Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China Department of Dermatology, Shandong Provincial Qianfoshan Hospital Affiliated to Shandong University, Jinan, Shandong 250014, P.R. China AU - Wang,Xiaoqing AU - Du,Guoqiang AU - Wu,Yidi AU - Zhang,Yongfei AU - Guo,Feng AU - Liu,Wei AU - Wu,Rongde DA - 2020/02/01 DO - 10.3892/ijo.2019.4948 EP - 580 IS - 2 JO - Int J Oncol KW - Wilms' tumor lipid metabolism fatty acid synthase mass spectrometry prognosis PY - 2020 SN - 1019-6439 1791-2423 SP - 568 ST - Association between different levels of lipid metabolism‑related enzymes and fatty acid synthase in Wilms' tumor T2 - International Journal of Oncology TI - Association between different levels of lipid metabolism‑related enzymes and fatty acid synthase in Wilms' tumor UR - https://doi.org/10.3892/ijo.2019.4948 VL - 56 ER -