TY - JOUR AB - Radiation therapy (RT) is an essential component in the therapeutic treatment of patients with localized prostate cancer (LPCa). Besides its local effects, ionizing radiation has been linked to mechanisms leading to systemic immune activation. The present study explored the effect of RT on the T‑cell receptor variable β (TCR Vβ) chain repertoire of peripheral blood T cells in patients with LPCa. High‑throughput TCR Vβ sequencing was performed on 20 blood samples collected from patients with LPCa at baseline and 3 months post‑RT. The diversity index was altered, as were TCR Vβ clonal evenness and convergence before and post‑RT; however, these findings were not significant. Notably, marked changes in the frequencies among the top 10 TCR Vβ clonotypes were detected and some patients developed new clonotypes of high abundance. These data provided initial evidence that RT in patients with LPCa may induce systemic immune changes, which could be exploited by future therapies for improved clinical results. AD - Cancer Immunology and Immunotherapy Center, Saint Savas Cancer Hospital, 11522 Athens, Greece ANTISEL S.A., 11527 Athens, Greece Biomedical Applications Unit, Institute of Chemical Biology, National Hellenic Research Foundation, 11635 Athens, Greece Department of Radiation Oncology, Saint Savas Cancer Hospital, 11522 Athens, Greece AU - Goulielmaki,Maria AU - Davanos,Nikolaos AU - Kogionou,Paraskevi AU - Batsaki,Panagiota AU - Stokidis,Savvas AU - Adamaki,Maria AU - Mosa,Eftychia AU - Vasilakou,Evanthia AU - Zambatis,Charalambos AU - Gritzapis,Angelos,D. AU - Zoumpourlis,Vassilios AU - Baxevanis,Constantin,N. AU - Fortis,Sotirios,P. AU - Goulielmaki,Maria AU - Davanos,Nikolaos AU - Kogionou,Paraskevi AU - Batsaki,Panagiota AU - Stokidis,Savvas AU - Adamaki,Maria AU - Mosa,Eftychia AU - Vasilakou,Evanthia AU - Zambatis,Charalambos AU - Gritzapis,Angelos,D. AU - Zoumpourlis,Vassilios AU - Baxevanis,Constantin,N. AU - Fortis,Sotirios,P. DA - 2022/06/01 DO - 10.3892/ijo.2022.5361 IS - 6 JO - Int J Oncol KW - T cell T‑cell receptor variable β prostate cancer radiation therapy clonotype frequencies PY - 2022 SN - 1019-6439 1791-2423 SP - 71 ST - The impact of radiation therapy on the TCR Vβ chain repertoire in patients with prostate cancer T2 - International Journal of Oncology TI - The impact of radiation therapy on the TCR Vβ chain repertoire in patients with prostate cancer UR - https://doi.org/10.3892/ijo.2022.5361 VL - 60 ER -