TY - JOUR AB - Thymoquinone (TQ) is likely responsible for the chemotherapeutic effects of N. sativa extract; however, the cellular mechanisms remain ill-defined. TQ-induced cytotoxicity was investigated using canine osteosarcoma (COS31), its cisplatin-resistant variant (COS31/rCDDP), human breast adenocarcinoma (MCF7), human ovarian adenocarcinoma (BG-1) and Madin-Darby canine (MDCK) cell lines. TQ-induced cytotoxicity was determined using a proliferation assay (MTT assay) and apoptosis assays. Effects of TQ on the cell cycle were determined using flow cytometry. COS31/rCDDP resistant cells were the most sensitive cell line to TQ and MDCK cells were the least sensitive. TQ (25 µM) induced apoptosis of COS31 cells 6 h after treatment and decreased the number of COS31 cells in S-phase and increased cells in G1-phase, indicating cell cycle arrest at G1. These results suggest that TQ kills cancer cells by a process that involves apoptosis and cell cycle arrest. Non-cancerous cells are relatively resistant to TQ. AD - Department of Pathology, College of Veterinary Medicine, The University of Tennessee, Knoxville, TN 37996-4543, USA null AU - Shoieb,Ahmed,M. AU - Elgayyar,Mona AU - Dudrick,Paul,S. AU - Bell,John,L. AU - Tithof,Patricia,K. DA - 2003/01/01 DO - 10.3892/ijo.22.1.107 EP - 113 IS - 1 JO - Int J Oncol PY - 2003 SN - 1019-6439 1791-2423 SP - 107 ST - In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone T2 - International Journal of Oncology TI - In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone UR - https://doi.org/10.3892/ijo.22.1.107 VL - 22 ER -