TY - JOUR AB - Thioredoxin (Trx), a small redox protein, is overexpressed in a number of tumors, however, its roles in tumor cells were not defined well. To investigate the effect of Trx, we transfected Trx or antisense Trx vectors into HT1080 cells. Trx-overexpressed HT1080 cells induced migration of endothelial cells through Flt-1 vascular endothelial growth factor (VEGF) receptor type-1. However, the migration was reduced by overexpression of antisense-Trx. To understand the relationship between Trx and hypoxia-induced angiogenesis, we observed the expression level of VEGF and hypoxia-inducible factor-1α (HIF-1α) after transfection of Trx. Overexpression of Trx also caused a significant increase of VEGF in protein and RNA levels under normoxic and hypoxic conditions. Moreover, transfection of Trx caused a dramatic increase in HIF-1α protein level under hypoxic condition. However, transfection of antisense-Trx markedly decreased HIF-1α and VEGF expression compared with controls. In addition, HIF-1α was not translocated from cytoplasm to nucleus in antisense-Trx overexpressing HT1080 cells. Further, we could detect the association of HIF-1α and pVHL in antisense-Trx transfectant HT1080 cells. Taken together, we suggest that Trx plays an important role in angiogenesis and, therefore, antisense-Trx might be applicable to the inhibition of tumor angiogenesis through the induction of pVHL-mediated HIF-1α degradation. AD - Department of Molecular Biology, Pusan National University, Busan 609-735, Korea null AU - Kim,Woo,Jean AU - Cho,Heeyeong AU - Lee,Sae-Won AU - Kim,Yung-Jin AU - Kim,Kyu-Won DA - 2005/04/01 DO - 10.3892/ijo.26.4.1049 EP - 1052 IS - 4 JO - Int J Oncol PY - 2005 SN - 1019-6439 1791-2423 SP - 1049 ST - Antisense-thioredoxin inhibits angiogenesis via pVHL-mediated hypoxia-inducible factor-1α degradation T2 - International Journal of Oncology TI - Antisense-thioredoxin inhibits angiogenesis via pVHL-mediated hypoxia-inducible factor-1α degradation UR - https://doi.org/10.3892/ijo.26.4.1049 VL - 26 ER -