TY - JOUR AB - Antisense transcription has long been recognized as a mechanism involved in the regulation of gene expression. Therefore, several human diseases associated with abnormal patterns of gene expression might display antisense RNA-mediated pathogenetic mechanisms. Such issue could be particularly relevant for cancer pathogenesis, since deregulated gene expression has long been established as a hallmark of cancer cells. Herein, we report on a bioinformatic search for antisense transcription in two cancer-associated regions of human chromosome 6 (6q21 and 6q27). Natural antisense transcripts (NATs) for several genes in both genomic regions were predicted in silico and subsequently validated by strand-specific RT-PCR. Detailed experimental validation by quantitative real-time RT-PCR of five putative cancer related sense-antisense transcript pairs revealed a single candidate tumor suppressor gene (RPS6KA2) whose expression levels display marked cancer-related changes that are likely mediated by its antisense RNA in a breast cancer cell line model. AD - Department of Biotechnology and Molecular Sciences, University of Insubria, I-21100 Varese, Italy null AU - Monti,Laura AU - Cinquetti,Raffaella AU - Guffanti,Alessandro AU - Nicassio,Francesco AU - Cremona,Mattia AU - Lavorgna,Giovanni AU - Bianchi,Fabrizio AU - Vignati,Francesca AU - Cittaro,Davide AU - Taramelli,Roberto AU - Acquati,Francesco DA - 2009/04/01 DO - 10.3892/ijo_00000237 EP - 1108 IS - 4 JO - Int J Oncol PY - 2009 SN - 1019-6439 1791-2423 SP - 1099 ST - In silico prediction and experimental validation of natural antisense transcripts in two cancer-associated regions of human chromosome 6 T2 - International Journal of Oncology TI - In silico prediction and experimental validation of natural antisense transcripts in two cancer-associated regions of human chromosome 6 UR - https://doi.org/10.3892/ijo_00000237 VL - 34 ER -