TY - JOUR AB - The clinical use of gene therapy requires tight regulation of the gene of interest and functional expression only when it is needed. Thus, it is necessary to develop ways of regulating functional gene expression with exogenous stimuli. Many regulatable systems are currently under development. For example, the tetracycline-dependent transcriptional switch has been successfully employed for in vivo preclinical applications. However, there are no examples of regulatable systems that have been employed in human clinical trials. In the present study, we established an adenovirus-delivered functional gene expression system that is regulated by estrogen. This system uses p16INK4A fused at its C-terminus to the ligand-binding domain of the estrogen receptor (ΔERα). We were able to establish cell lines expressing this gene wherein the functional expression of p16INK4A is estrogen-dependent and causes the arrest of several ovarian cancer cell lines. This inducible and adenovirus-mediated gene transfer system may allow gene therapy using nuclear functioning genes in postmenopausal or ovariectomized women. AD - Department of Gynecology and Reproductive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan null AU - Tamura,Tomohiro AU - Kanuma,Tatsuya AU - Nakazato,Tomoko AU - Faried,Leri,S. AU - Aoki,Hiroshi AU - Minegishi,Takashi DA - 2010/04/01 DO - 10.3892/ijo_00000569 EP - 912 IS - 4 JO - Int J Oncol PY - 2010 SN - 1019-6439 1791-2423 SP - 905 ST - A new system for regulated functional gene expression for gene therapy applications: Nuclear delivery of a p16INK4A-estrogen receptor carboxy terminal fusion protein only in the presence of estrogen T2 - International Journal of Oncology TI - A new system for regulated functional gene expression for gene therapy applications: Nuclear delivery of a p16INK4A-estrogen receptor carboxy terminal fusion protein only in the presence of estrogen UR - https://doi.org/10.3892/ijo_00000569 VL - 36 ER -